PDBsum entry 2ar0

Transcription,transferase

PDB id: 2ar0

Contents

Protein chains

485 a.a. *

Ligands

UNX ×10

Waters ×143

* Residue conservation analysis

PDB id:

2ar0

Name:

Transcription,transferase

Title:

Crystal structure of type i restriction enzyme ecoki m protein (ec 2.1.1.72) (m.Ecoki)

Structure:

Type i restriction enzyme ecoki m protein. Chain: a, b. Synonym: m.Ecoki. Engineered: yes. Mutation: yes

Source:

Escherichia coli. Organism_taxid: 562. Gene: hsdm, hsm. Expressed in: escherichia coli. Expression_system_taxid: 562.

Resolution:

2.80Å R-factor: 0.224 R-free: 0.261

Authors:

K.R.Rajashankar,R.Kniewel,C.D.Lima,S.K.Burley,New York Sgx Research Center For Structural Genomics (Nysgxrc)

Key ref:

K.R.Rajashankar et al. Crystal structure of type I restriction enzyme ecoki m protein (ec 2.1.1.72) (m.Ecoki).. To be published, .

Date:

18-Aug-05 Release date: 30-Aug-05

Protein chains

P08957  (T1MK_ECOLI) -  Type I restriction enzyme EcoKI methylase subunit from Escherichia coli (strain K12)

Seq: 529 a.a.

Struc: 485 a.a.

Enzyme reactions

• Enzyme class: E.C.2.1.1.72 - site-specific DNA-methyltransferase (adenine-specific).
• Reaction: a 2'-deoxyadenosine in DNA + S-adenosyl-L-methionine = an N₆-methyl- 2'-deoxyadenosine in DNA + S-adenosyl-L-homocysteine + H⁺

Molecule diagrams generated from .mol files obtained from the KEGG ftp site