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PDBsum entry 2x72
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protein ligands Protein-protein interface(s) links
Signaling protein PDB id
2x72

 

 

 

 

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Contents
Protein chains
327 a.a.
11 a.a.
Ligands
NAG-NAG-BMA-MAN-
MAN
PEF
BOG
PLM ×2
RET
LPP
ACT
Waters ×18
PDB id:
2x72
Name: Signaling protein
Title: Crystal structure of the constitutively active e113q,d2c,d282c rhodopsin mutant with bound galphact peptide.
Structure: Rhodopsin. Chain: a. Engineered: yes. Mutation: yes. Guanine nucleotide-binding protein g(t) subunit alpha-1. Chain: b. Fragment: residues 340-350. Synonym: gact peptide, transducin alpha-1 chain. Engineered: yes.
Source: Bos taurus. Cattle. Organism_taxid: 9913. Organ: eye. Tissue: retina. Cell: rod photoreceptor. Expressed in: homo sapiens. Expression_system_taxid: 9606. Expression_system_cell_line: hek293s gnti- cells.
Resolution:
3.00Å     R-factor:   0.212     R-free:   0.244
Authors: J.Standfuss,P.C.Edwards,A.Dantona,M.Fransen,G.Xie,D.D.Oprian, G.F.X.Schertler
Key ref: J.Standfuss et al. (2011). The structural basis of agonist-induced activation in constitutively active rhodopsin. Nature, 471, 656-660. PubMed id: 21389983
Date:
22-Feb-10     Release date:   16-Mar-11    
PROCHECK
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 Headers
 References

Protein chain
Pfam   ArchSchema ?
P02699  (OPSD_BOVIN) -  Rhodopsin from Bos taurus
Seq:
Struc: 		348 a.a.
326 a.a.*
Protein chain
Pfam   ArchSchema ?
P04695  (GNAT1_BOVIN) -  Guanine nucleotide-binding protein G(t) subunit alpha-1 from Bos taurus
Seq:
Struc: 		350 a.a.
11 a.a.*
Key:    PfamA domain  Secondary structure  CATH domain
* PDB and UniProt seqs differ at 4 residue positions (black crosses)

 

 
Nature 471:656-660 (2011)
PubMed id: 21389983  
 
 
The structural basis of agonist-induced activation in constitutively active rhodopsin.
J.Standfuss, P.C.Edwards, A.D'Antona, M.Fransen, G.Xie, D.D.Oprian, G.F.Schertler.
 
  ABSTRACT  
 
No abstract given.

 

Literature references that cite this PDB file's key reference

  PubMed id Reference
23407534 A.J.Venkatakrishnan, X.Deupi, G.Lebon, C.G.Tate, G.F.Schertler, and M.M.Babu (2013).
Molecular signatures of G-protein-coupled receptors.
  Nature, 494, 185-194.  
22437504 H.Wu, D.Wacker, M.Mileni, V.Katritch, G.W.Han, E.Vardy, W.Liu, A.A.Thompson, X.P.Huang, F.I.Carroll, S.W.Mascarella, R.B.Westkaemper, P.D.Mosier, B.L.Roth, V.Cherezov, and R.C.Stevens (2012).
Structure of the human κ-opioid receptor in complex with JDTic.
  Nature, 485, 327-332.
PDB code: 4djh
23051748 J.F.White, N.Noinaj, Y.Shibata, J.Love, B.Kloss, F.Xu, J.Gvozdenovic-Jeremic, P.Shah, J.Shiloach, C.G.Tate, and R.Grisshammer (2012).
Structure of the agonist-bound neurotensin receptor.
  Nature, 490, 508-513.
PDB code: 4grv
23237917 R.C.Stevens, V.Cherezov, V.Katritch, R.Abagyan, P.Kuhn, H.Rosen, and K.Wüthrich (2012).
The GPCR Network: a large-scale collaboration to determine human GPCR structure and function.
  Nat Rev Drug Discov, 12, 25-34.  
21956331 K.Y.Chung, S.G.Rasmussen, T.Liu, S.Li, B.T.DeVree, P.S.Chae, D.Calinski, B.K.Kobilka, V.L.Woods, and R.K.Sunahara (2011).
Conformational changes in the G protein Gs induced by the β2 adrenergic receptor.
  Nature, 477, 611-615.  
21772288 S.G.Rasmussen, B.T.DeVree, Y.Zou, A.C.Kruse, K.Y.Chung, T.S.Kobilka, F.S.Thian, P.S.Chae, E.Pardon, D.Calinski, J.M.Mathiesen, S.T.Shah, J.A.Lyons, M.Caffrey, S.H.Gellman, J.Steyaert, G.Skiniotis, W.I.Weis, R.K.Sunahara, and B.K.Kobilka (2011).
Crystal structure of the β2 adrenergic receptor-Gs protein complex.
  Nature, 477, 549-555.
PDB code: 3sn6
The most recent references are shown first. Citation data come partly from CiteXplore and partly from an automated harvesting procedure. Note that this is likely to be only a partial list as not all journals are covered by either method. However, we are continually building up the citation data so more and more references will be included with time. Where a reference describes a PDB structure, the PDB code is shown on the right.

 

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