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PDBsum entry 1vpd
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Oxidoreductase
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PDB id
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1vpd
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References listed in PDB file
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Key reference
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Title
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X-Ray crystal structure of garr-Tartronate semialdehyde reductase from salmonella typhimurium.
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Authors
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J.Osipiuk,
M.Zhou,
S.Moy,
F.Collart,
A.Joachimiak.
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Ref.
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J Struct Funct Genomics, 2009,
10,
249-253.
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PubMed id
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Abstract
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Tartronate semialdehyde reductases (TSRs), also known as
2-hydroxy-3-oxopropionate reductases, catalyze the reduction of tartronate
semialdehyde using NAD as cofactor in the final stage of D-glycerate
biosynthesis. These enzymes belong to family of structurally and mechanically
related beta-hydroxyacid dehydrogenases which differ in substrate specificity
and catalyze reactions in specific metabolic pathways. Here, we present the
crystal structure of GarR a TSR from Salmonella typhimurium determined by the
single-wavelength anomalous diffraction method and refined to 1.65 A resolution.
The active site of the enzyme contains L-tartrate which most likely mimics a
position of a glycerate which is a product of the enzyme reaction. The analysis
of the TSR structure shows also a putative NADPH binding site in the enzyme.
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