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PDBsum entry 1vcz
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References listed in PDB file
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Key reference
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Title
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Crystal structures of the nicotiana glutinosa ribonuclease nt in complex with nucleoside monophosphates.
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Authors
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S.Kawano,
Y.Kakuta,
T.Nakashima,
M.Kimura.
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Ref.
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J Biochem (tokyo), 2006,
140,
375-381.
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PubMed id
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Note In the PDB file this reference is
annotated as "TO BE PUBLISHED".
The citation details given above were identified by an automated
search of PubMed on title and author
names, giving a
percentage match of
94%.
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Abstract
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Ribonuclease NT (RNase NT), induced upon tobacco mosaic virus (TMV) infection in
Nicotiana glutinosa leaves, has a broad base specificity. The crystal structures
of RNase NT in complex with either 5'-AMP, 5'-GMP, or 2'-UMP were determined at
1.8 A resolutions by molecular replacement. RNase NT consists of seven helices
and seven beta strands, and the structure is highly similar to that of RNase NW,
a guanylic acid preferential RNase from the N. glutinosa leaves, showing root
mean square deviation (rmsd) of 1.1 A over an entire length of two molecules for
Calpha atoms. The complex structures revealed that Trp42, Asn44, and Trp50 are
involved in interactions with bases at B1 site (primary site), whereas Gln12,
Tyr17, Ser78, Leu79, and Phe89 participate in recognition of bases at B2 site
(subsite). The 5'-GMP and 5'-AMP bind both B1 and B2 sites in RNase NT, while
2'-UMP predominantly binds B1 site in the complex. The nucleotide binding modes
in these complexes would provide a clue to elucidation of structural basis for
the broad base specificity for RNase NT.
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