 |
PDBsum entry 1sb3
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
 |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
Oxidoreductase
|
PDB id
|
|
|
|
1sb3
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
 |
Contents |
 |
|
|
|
|
|
|
|
|
|
|
|
761 a.a.
|
|
|
|
|
|
|
|
|
|
|
323 a.a.
|
|
|
|
|
|
|
|
|
|
|
161 a.a.
|
|
|
|
|
|
|
|
|
|
|
|
|
|
References listed in PDB file
|
|
|
Key reference
|
|
|
Title
|
|
Structure of a xanthine oxidase-Related 4-Hydroxybenzoyl-Coa reductase with an additional [4fe-4s] cluster and an inverted electron flow.
|
|
|
Authors
|
|
M.Unciuleac,
E.Warkentin,
C.C.Page,
M.Boll,
U.Ermler.
|
|
|
Ref.
|
|
Structure, 2004,
12,
2249-2256.
[DOI no: ]
|
|
|
PubMed id
|
|
|
|
|
|
Abstract
|
|
|
The Mo-flavo-Fe/S-dependent heterohexameric protein complex 4-hydroxybenzoyl-CoA
reductase (4-HBCR, dehydroxylating) is a central enzyme of the anaerobic
degradation of phenolic compounds and belongs to the xanthine oxidase (XO)
family of molybdenum enzymes. Its X-ray structure was established at 1.6 A
resolution. The most pronounced difference between 4-HBCR and other structurally
characterized members of the XO family is the insertion of 40 amino acids within
cluster at a distance of
16.5 A to the isoalloxazine ring of FAD. The architecture of 4-HBCR and
concomitantly performed electron transfer rate calculations suggest an inverted
cluster to
the Mo over a distance of 55 A. The binding site of 4-hydroxybenzoyl-CoA is
located in an 18 A long channel lined up by several aromatic side chains around
the aromatic moiety, which are proposed to shield and stabilize the postulated
radical intermediates during catalysis.
|
|
|
|
|
|
Figure 1.
Figure 1. Reaction Catalyzed by 4-Hydroxybenzoyl-CoA
Reductase
|
|
|
|
|
|
The above figure is
reprinted
by permission from Cell Press:
Structure
(2004,
12,
2249-2256)
copyright 2004.
|
|
|
Secondary reference #1
|
|
|
Title
|
|
Crystallization of 4-Hydroxybenzoyl-Coa reductase and the structure of its electron donor ferredoxin.
|
|
|
Authors
|
|
M.Unciuleac,
M.Boll,
E.Warkentin,
U.Ermler.
|
|
|
Ref.
|
|
Acta Crystallogr D Biol Crystallogr, 2004,
60,
388-391.
[DOI no: ]
|
|
|
PubMed id
|
|
|
|
|
|
|
|
|
|
|
|
Figure 1.
Figure 1 Reactions catalyzed by benzoyl-CoA reductase (1) and
4-hydroxybenzoyl-CoA reductase (2). In T. aromatica, reduced
ferredoxin serves as in vivo electron donor for both reactions;
it is regenerated by 2-oxoglutarate:ferredoxin oxidoreductase.
|
|
|
|
|
|
The above figure is
reproduced from the cited reference
with permission from the IUCr
|
|
|
|
|
|
|
