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PDBsum entry 1r56
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Oxidoreductase
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PDB id
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1r56
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Contents |
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* Residue conservation analysis
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References listed in PDB file
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Key reference
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Title
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Complexed and ligand-Free high-Resolution structures of urate oxidase (uox) from aspergillus flavus: a reassignment of the active-Site binding mode.
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Authors
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P.Retailleau,
N.Colloc'H,
D.Vivarès,
F.Bonneté,
B.Castro,
M.El-Hajji,
J.P.Mornon,
G.Monard,
T.Prangé.
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Ref.
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Acta Crystallogr D Biol Crystallogr, 2004,
60,
453-462.
[DOI no: ]
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PubMed id
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Abstract
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High-resolution X-ray structures of the complexes of Aspergillus flavus urate
oxidase (Uox) with three inhibitors, 8-azaxanthin (AZA), 9-methyl uric acid
(MUA) and oxonic acid (OXC), were determined in an orthorhombic space group
(I222). In addition, the ligand-free enzyme was also crystallized in a
monoclinic form (P2(1)) and its structure determined. Higher accuracy in the
three new enzyme-inhibitor complex structures (Uox-AZA, Uox-MUA and Uox-OXC)
with respect to the previously determined structure of Uox-AZA (PDB code 1uox)
leads to a reversed position of the inhibitor in the active site of the enzyme.
The corrected anchoring of the substrate (uric acid) allows an improvement in
the understanding of the enzymatic mechanism of urate oxidase.
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Figure 1.
Figure 1 Schematic degradation pathway of uric acid to allantoin
through intermediate (2) (5-hydroxyisourate). Only the first
step is catalyzed by the enzyme, with H[2]O[2] as the by-product.
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Figure 7.
Figure 7 Revised position of 8-azaxanthin relative to the active
site of Uox. (a) Original published location of the AZA
inhibitor in [212]1uox ; (b) the revised orientation of AZA in
the new refined structure (1.8 Å). Both orientations are
superimposed on the respective final 2|F[obs]| - |F[calc]|
electron-density maps contoured around the inhibitor at 1.75
[213][sigma] .
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The above figures are
reprinted
by permission from the IUCr:
Acta Crystallogr D Biol Crystallogr
(2004,
60,
453-462)
copyright 2004.
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Secondary reference #1
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Title
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Crystal structure of the protein drug urate oxidase-Inhibitor complex at 2.05 a resolution.
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Authors
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N.Colloc'H,
M.El hajji,
B.Bachet,
G.L'Hermite,
M.Schiltz,
T.Prangé,
B.Castro,
J.P.Mornon.
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Ref.
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Nat Struct Biol, 1997,
4,
947-952.
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PubMed id
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