PDBsum entry 1pg8

Lyase

PDB id: 1pg8

Contents

Protein chains

398 a.a. *

Ligands

SO4 ×4

PLP ×4

PEG ×11

Waters ×424

* Residue conservation analysis

PDB id:

1pg8

Name:

Lyase

Title:

Crystal structure of l-methionine alpha-, gamma-lyase

Structure:

Methionine gamma-lyase. Chain: a, b, c, d. Synonym: l-methioninase. Ec: 4.4.1.11

Source:

Pseudomonas putida. Organism_taxid: 303

Biol. unit:

Octamer (from PQS)

Resolution:

2.68Å R-factor: 0.182 R-free: 0.236

Authors:

T.W.Allen,V.Sridhar,G.S.Prasad,Q.Han,M.Xu,Y.Tan,R.M.Hoffman, S.Ramaswamy

Date:

28-May-03 Release date: 08-Jun-04

Protein chains

P13254  (MEGL_PSEPU) -  L-methionine gamma-lyase from Pseudomonas putida

Seq: 398 a.a.

Struc: 398 a.a.

Enzyme reactions

• Enzyme class 1: E.C.4.4.1.11 - methionine gamma-lyase.
• Reaction: L-methionine + H2O = methanethiol + 2-oxobutanoate + NH4⁺

L-methionine + H2O = methanethiol (Bound ligand (Het Group name = PEG) matches with 40.00% similarity) + 2-oxobutanoate + NH4(+)

• Cofactor: Pyridoxal 5'-phosphate

Pyridoxal 5'-phosphate (Bound ligand (Het Group name = PLP) matches with 93.75% similarity)

• Enzyme class 2: E.C.4.4.1.2 - homocysteine desulfhydrase.
• Reaction: L-homocysteine + H2O = 2-oxobutanoate + hydrogen sulfide + NH4⁺ + H⁺

L-homocysteine + H2O = 2-oxobutanoate (Bound ligand (Het Group name = PEG) matches with 40.00% similarity) + hydrogen sulfide + NH4(+) + H(+)

• Cofactor: Pyridoxal 5'-phosphate

Pyridoxal 5'-phosphate (Bound ligand (Het Group name = PLP) matches with 93.75% similarity)

Note, where more than one E.C. class is given (as above), each may correspond to a different protein domain or, in the case of polyprotein precursors, to a different mature protein.

Molecule diagrams generated from .mol files obtained from the KEGG ftp site