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PDBsum entry 1pd1
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Transport protein
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PDB id
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1pd1
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Contents |
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* Residue conservation analysis
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References listed in PDB file
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Key reference
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Title
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Snare selectivity of the copii coat.
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Authors
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E.Mossessova,
L.C.Bickford,
J.Goldberg.
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Ref.
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Cell, 2003,
114,
483-495.
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PubMed id
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Abstract
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The COPII coat buds transport vesicles from the endoplasmic reticulum that
incorporate cargo and SNARE molecules. Here, we show that recognition of the
ER-Golgi SNAREs Bet1, Sed5, and Sec22 occurs through three binding sites on the
Sec23/24 subcomplex of yeast COPII. The A site binds to the YNNSNPF motif of
Sed5. The B site binds to Lxx-L/M-E sequences present in both the Bet1 and Sed5
molecules, as well as to the DxE cargo-sorting signal. A third, spatially
distinct site binds to Sec22. COPII selects the free v-SNARE form of Bet1
because the LxxLE sequence is sequestered in the four-helix bundle of the
v-/t-SNARE complex. COPII favors Sed5 within the Sed5/Bos1/Sec22 t-SNARE complex
because t-SNARE assembly removes autoinhibitory contacts to expose the YNNSNPF
motif. The COPII coat seems to be a specific conductor of the fusogenic forms of
these SNAREs, suggesting how vesicle fusion specificity may be programmed during
budding.
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