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PDBsum entry 1llm
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Transcription/DNA
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PDB id
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1llm
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Contents |
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* Residue conservation analysis
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References listed in PDB file
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Key reference
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Title
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Structure of a designed dimeric zinc finger protein bound to DNA.
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Authors
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S.A.Wolfe,
R.A.Grant,
C.O.Pabo.
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Ref.
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Biochemistry, 2003,
42,
13401-13409.
[DOI no: ]
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PubMed id
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Abstract
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Proteins that employ dimerization domains to bind cooperatively to DNA have a
number of potential advantages over monomers with regards to gene regulation.
Using a combination of structure-based design and phage display, a dimeric
Cys(2)His(2) zinc finger protein has been created that binds cooperatively to
DNA via an attached leucine zipper dimerization domain. This chimera, derived
from components of Zif268 and GCN4, displayed excellent DNA-binding specificity,
and we now report the 1.5 A resolution cocrystal structure of the Zif268-GCN4
homodimer bound to DNA. This structure shows how phage display has annealed the
DNA binding and dimerization domains into a single functional unit. Moreover,
this chimera provides a potential platform for the creation heterodimeric zinc
finger proteins that can regulate a desired target gene through cooperative DNA
recognition.
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Secondary reference #1
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Title
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Combining structure-Based design with phage display to create new cys(2)his(2) zinc finger dimers.
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Authors
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S.A.Wolfe,
E.I.Ramm,
C.O.Pabo.
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Ref.
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Structure, 2000,
8,
739-750.
[DOI no: ]
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PubMed id
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Figure 7.
Figure 7. Zif23-GCN4( -2) fused to the VP16 activation
domain activates a promoter containing the -2 site in vivo. The
normalized luciferase activity for each combination of
expression and reporter vectors is indicated as a vertical bar.
Each value represents the average of three to five experiments
with the standard error of the mean indicated as a vertical line
near the top of each bar. (a) Normalized luciferase activity
observed from various reporters when transfected into 293 cells
with the Zif23-GCN4( -2)-VP16 expression vector. The identity of
the binding sites in each reporter is indicated below each
column. The pGL2 control refers to the parent reporter vector
without any binding sites inserted. (b) Normalized luciferase
activity observed from the -2 site reporter when transfected
into 293 cells with the various expression vectors. The identity
of each expression vector is indicated below each column. The
Rc-CMV2 control refers to the parent expression vector without a
gene inserted downstream of the CMV promoter.
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The above figure is
reproduced from the cited reference
with permission from Cell Press
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