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PDBsum entry 1kuq
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Contents |
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* Residue conservation analysis
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PDB id:
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Ribosome
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Title:
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Crystal structure of t3c mutant s15 ribosomal protein in complex with 16s rrna
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Structure:
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16s ribosomal RNA fragment. Chain: b. Engineered: yes. 30s ribosomal protein s15. Chain: a. Engineered: yes. Mutation: yes
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Source:
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Thermus thermophilus. Organism_taxid: 274. Expressed in: escherichia coli. Expression_system_taxid: 562.
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Biol. unit:
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Tetramer (from
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Resolution:
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2.84Å
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R-factor:
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0.231
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R-free:
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0.322
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Authors:
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A.D.Nikulin,S.Tishchenko,S.Revtovich,B.Ehresmann,C.Ehresmann,P.Dumas, M.Garber,S.Nikonov,N.Nevskaya
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Key ref:
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S.V.Revtovich
et al.
(2004).
Role of N-terminal helix in interaction of ribosomal protein S15 with 16S rRNA.
Biochemistry (mosc),
69,
1319-1323.
PubMed id:
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Date:
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22-Jan-02
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Release date:
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24-Jun-03
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PROCHECK
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Headers
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References
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P80378
(RS15_THETH) -
Small ribosomal subunit protein uS15 from Thermus thermophilus
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Seq:
Struc:
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89 a.a.
84 a.a.*
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Key: |
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PfamA domain |
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Secondary structure |
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CATH domain |
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*
PDB and UniProt seqs differ
at 1 residue position (black
cross)
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G-G-G-C-G-G-C-C-U-U-C-G-G-G-C-U-A-G-A-C-G-G-U-G-G-G-A-G-A-G-G-C-U-U-C-G-G-C-U-
57 bases
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Biochemistry (mosc)
69:1319-1323
(2004)
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PubMed id:
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Role of N-terminal helix in interaction of ribosomal protein S15 with 16S rRNA.
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S.V.Revtovich,
A.D.Nikulin,
S.V.Nikonov.
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ABSTRACT
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The position and conformation of the N-terminal helix of free ribosomal protein
S15 was earlier found to be modified under various conditions. This variability
was supposed to provide the recognition by the protein of its specific site on
16S rRNA. To test this hypothesis, we substituted some amino acid residues in
this helix and assessed effects of these substitutions on the affinity of the
protein for 16S rRNA. The crystal structure of the complex of one of these
mutants (Thr3Cys S15) with the 16S rRNA fragment was determined, and a computer
model of the complex containing another mutant (Gln8Met S15) was designed. The
available and new information was analyzed in detail, and the N-terminal helix
was concluded to play no significant role in the specific binding of the S15
protein to its target on 16S rRNA.
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Literature references that cite this PDB file's key reference
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PubMed id
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Reference
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T.Créty,
and
T.E.Malliavin
(2007).
The conformational landscape of the ribosomal protein S15 and its influence on the protein interaction with 16S RNA.
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Biophys J,
92,
2647-2665.
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PDB code:
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The most recent references are shown first.
Citation data come partly from CiteXplore and partly
from an automated harvesting procedure. Note that this is likely to be
only a partial list as not all journals are covered by
either method. However, we are continually building up the citation data
so more and more references will be included with time.
Where a reference describes a PDB structure, the PDB
code is
shown on the right.
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Links
