PDBsum entry 1kq4

Transferase

PDB id: 1kq4

Contents

Protein chains

203 a.a. *

214 a.a. *

Ligands

FAD ×4

Waters ×274

* Residue conservation analysis

References listed in PDB file

Key reference

Title

Structural genomics of the thermotoga maritima proteome implemented in a high-Throughput structure determination pipeline.

Authors

S.A.Lesley, P.Kuhn, A.Godzik, A.M.Deacon, I.Mathews, A.Kreusch, G.Spraggon, H.E.Klock, D.Mcmullan, T.Shin, J.Vincent, A.Robb, L.S.Brinen, M.D.Miller, T.M.Mcphillips, M.A.Miller, D.Scheibe, J.M.Canaves, C.Guda, L.Jaroszewski, T.L.Selby, M.A.Elsliger, J.Wooley, S.S.Taylor, K.O.Hodgson, I.A.Wilson, P.G.Schultz, R.C.Stevens.

Ref.

Proc Natl Acad Sci U S A, 2002, 99, 11664-11669. [DOI no: 10.1073/pnas.142413399]

PubMed id

12193646

Abstract

Structural genomics is emerging as a principal approach to define protein structure-function relationships. To apply this approach on a genomic scale, novel methods and technologies must be developed to determine large numbers of structures. We describe the design and implementation of a high-throughput structural genomics pipeline and its application to the proteome of the thermophilic bacterium Thermotoga maritima. By using this pipeline, we successfully cloned and attempted expression of 1,376 of the predicted 1,877 genes (73%) and have identified crystallization conditions for 432 proteins, comprising 23% of the T. maritima proteome. Representative structures from TM0423 glycerol dehydrogenase and TM0449 thymidylate synthase-complementing protein are presented as examples of final outputs from the pipeline.

Figure 1.
Fig. 1. T. maritima structural genomics pipeline.

Figure 2.
Fig. 2. Custom instrumentation used to process T. maritima proteome. (A) A 96-tube fermentor for high-cell density growth. (B) Purification robot that processes cell pellets from fermentation through affinity purification. (C) Nano-drop crystallization robotics used to set up crystal trials. (D) Plate imaging robotics used to analyze crystal trials.