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PDBsum entry 1jw6
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Sugar binding protein
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PDB id
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1jw6
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Contents |
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* Residue conservation analysis
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References listed in PDB file
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Key reference
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Title
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Crystal structure of the complex of concanavalin a and hexapeptide.
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Authors
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Z.Zhang,
M.Qian,
Q.Huang,
Y.Jia,
Y.Tang,
K.Wang,
D.Cui,
M.Li.
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Ref.
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J Protein Chem, 2001,
20,
423-429.
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PubMed id
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Note: In the PDB file this reference is
annotated as "TO BE PUBLISHED". The citation details given above have
been manually determined.
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Abstract
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The X-ray structure analysis of a cross-linked crystal of concanavalin A soaked
with a hexapeptide molecule as a probe molecule showed an electron density
corresponding to full occupation in the binding pocket. The site lies on the
surface of concanavalin A and is surrounded by three symmetry-related molecules.
The crystal structure of the hexapeptide complex was refined at 1.93-A
resolution, to an R-factor of 19% (Rfree factor of 25%), with an RMS deviation
in bond distances of 0.01 A. The model includes all 237 residues of concanavalin
A, one manganese ion, one calcium ion, 95 water molecules, one glutaraldehyde
molecule, one isopropanol molecule, and one hexapeptide molecule. This X-ray
structure analysis also provides an approach to mapping the binding surface of
crystalline protein with a probe molecule that is dissolved in the mixture of
organic solvent with water or in neat organic solvent but is hardly dissolved in
aqueous solution.
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Secondary reference #1
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Title
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Crystal structure of the complex of concanavalin a and tripeptide.
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Authors
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Z.Zhang,
M.Qian,
Q.Huang,
Y.Jia,
Y.Tang,
K.Wang,
D.Cui,
M.Li.
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Ref.
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J Protein Chem, 2001,
20,
59-65.
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PubMed id
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