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PDBsum entry 1dcl
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Immunoglobulin
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PDB id
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1dcl
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Contents |
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* Residue conservation analysis
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References listed in PDB file
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Key reference
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Title
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Three-Dimensional structure of a light chain dimer crystallized in water. Conformational flexibility of a molecule in two crystal forms.
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Authors
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K.R.Ely,
J.N.Herron,
M.Harker,
A.B.Edmundson.
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Ref.
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J Mol Biol, 1989,
210,
601-615.
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PubMed id
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Abstract
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The three-dimensional structure of an immunoglobulin light chain dimer (Mcg)
crystallized in deionized water (orthorhombic form) was determined at 2.0 A
resolution by phase extension and crystallographic refinement. This structure
was refined side-by-side with that of the same molecule crystallized in ammonium
sulfate (trigonal form). The dimer adopted markedly different structures in the
two solvents. "Elbow bend" angles between pseudo 2-fold axes of rotation
relating pairs of "variable" (V) and "constant" (C) domains were found to be 132
degrees in the orthorhombic form and 115 degrees in the trigonal form. Modes of
association of the V domains and, to a lesser extent, the pairing interactions
of the C domains were different in the two structures. Alterations in the V
domain pairing were reflected in the shapes of the binding regions and in the
orientations of the side-chains lining the walls of the binding sites. In the
trigonal form, for instance, the V domain interface was compartmentalized into a
main binding cavity and a deep pocket, whereas these spaces were continuous in
the orthorhombic structure. Patterns of ordered water molecules were quite
distinct in the two crystal types. In some cases, the solvent structures could
be correlated with conformational changes in the proteins. For example, close
contacts between V and C domains of monomer 1 of the trigonal form were not
retained in orthorhombic crystals. Ordered water molecules filled the space
created when the two domains moved apart.
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Secondary reference #1
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Title
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The refinement and structure of mcg bence-Jones dimer at 2.3 angstroms resolution
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Authors
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Z.B.Xu,
M.Schiffer.
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Ref.
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am cryst assoc ,abstr, 1988,
16,
74.
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Secondary reference #2
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Title
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Structure of a lambda-Type bence-Jones protein at 3.5-A resolution.
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Authors
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M.Schiffer,
R.L.Girling,
K.R.Ely,
A.B.Edmundson.
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Ref.
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Biochemistry, 1973,
12,
4620-4631.
[DOI no: ]
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PubMed id
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