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PDBsum entry 1dbm
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Immunoglobulin
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PDB id
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1dbm
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Contents |
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* Residue conservation analysis
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References listed in PDB file
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Key reference
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Title
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Molecular basis of crossreactivity and the limits of antibody-Antigen complementarity.
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Authors
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J.H.Arevalo,
M.J.Taussig,
I.A.Wilson.
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Ref.
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Nature, 1993,
365,
859-863.
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PubMed id
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Abstract
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Two major unanswered questions concerning the specificity of antibodies are: how
do structurally different antigens bind with high affinity to the same antibody,
and what are the limits of the antibody combining site complementarity and
flexibility that contribute to such crossreactivity? We report here a
comparative analysis of the X-ray structures of five conformationally different
steroids in complex with the Fab' fragment of an anti-progesterone antibody DB3
at 2.7 A. This antibody is unable to complement completely the shape of the
hydrophobic antigen so that crossreactivity occurs with other ligands without
major structural rearrangements of the binding site. Antigen specificity can be
explained through conserved interactions of DB3 with the steroid D-ring, whereas
some of the crossreactivity is realized through different binding orientations
of the steroid skeleton that place the A-ring into alternative pockets on the
antibody surface. The restricted gene usage of the VGAM3.8 family in the
generation of anti-progesterone monoclonal antibodies may be explained by the
specific interaction of VH hallmark residues with the steroid D-ring. This first
detailed structure of steroid interactions with a protein could be applied to
the understanding of general mechanisms of steroid recognition as well as in the
design of specific binding sites for small hydrophobic ligands.
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Secondary reference #1
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Title
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Structural analysis of antibody specificity. Detailed comparison of five FAB'-Steroid complexes.
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Authors
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J.H.Arevalo,
C.A.Hassig,
E.A.Stura,
M.J.Sims,
M.J.Taussig,
I.A.Wilson.
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Ref.
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J Mol Biol, 1994,
241,
663-690.
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PubMed id
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Note In the PDB file this reference is
annotated as "TO BE PUBLISHED".
The citation details given above were identified by an automated
search of PubMed on title and author
names, giving a
percentage match of
94%.
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Secondary reference #2
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Title
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Three-Dimensional structure of an anti-Steroid FAB' And progesterone-Fab' Complex.
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Authors
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J.H.Arevalo,
E.A.Stura,
M.J.Taussig,
I.A.Wilson.
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Ref.
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J Mol Biol, 1993,
231,
103-118.
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PubMed id
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Secondary reference #3
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Title
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Analysis of an anti-Progesterone antibody: variable crystal morphology of the FAB' And steroid-Fab' Complexes.
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Authors
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E.A.Stura,
J.H.Arevalo,
A.Feinstein,
R.B.Heap,
M.J.Taussig,
I.A.Wilson.
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Ref.
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Immunology, 1987,
62,
511-521.
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PubMed id
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