PDBsum entry 1c8j

Oxidoreductase

PDB id: 1c8j

Contents

Protein chains

405 a.a. *

Ligands

HEM ×2

Waters ×233

* Residue conservation analysis

PDB id:

1c8j

Name:

Oxidoreductase

Title:

Crystal structure of cytochrome p450cam mutant (f87w/y96f)

Structure:

Cytochrome p450-cam. Chain: a, b. Synonym: camphor 5-monooxygenase. Engineered: yes. Mutation: yes

Source:

Pseudomonas putida. Organism_taxid: 303. Expressed in: escherichia coli. Expression_system_taxid: 562.

Resolution:

2.10Å R-factor: 0.193 R-free: 0.264

Authors:

Y.Liu,F.Jiang,Q.Guo,X.Chen,J.Jin,Y.Sun,Z.Rao

Key ref:

Y.Liu et al. Crystal structure of cytochrome p450cam mutant (f87w/y96f). To be published, .

Date:

31-May-00 Release date: 09-May-01

Protein chains

P00183  (CPXA_PSEPU) -  Camphor 5-monooxygenase from Pseudomonas putida

Seq: 415 a.a.

Struc: 405 a.a.*

* PDB and UniProt seqs differ at 2 residue positions (black crosses)

Enzyme reactions

• Enzyme class: E.C.1.14.15.1 - camphor 5-monooxygenase.
• Reaction: 2 reduced [2Fe-2S]-[putidaredoxin] + (1R,4R)-camphor + O2 + 2 H⁺ = (1R,4R,5R)-5-hydroxycamphor + 2 oxidized [2Fe-2S]-[putidaredoxin] + H2O
• Cofactor: Heme-thiolate

Molecule diagrams generated from .mol files obtained from the KEGG ftp site