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PDBsum entry 1bcf
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Iron storage and electron transport
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PDB id
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1bcf
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Contents |
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* Residue conservation analysis
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References listed in PDB file
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Key reference
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Title
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Structure of a unique twofold symmetric haem-Binding site.
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Authors
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F.Frolow,
A.J.Kalb,
J.Yariv.
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Ref.
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Nat Struct Biol, 1994,
1,
453-460.
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PubMed id
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Abstract
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Bacterioferritin of Escherichia coli, also known as cytochrome b1, is a hollow,
nearly spherical shell made up of 24 identical protein subunits and 12 haems. We
have solved this structure in a tetragonal crystal form at 2.9 A resolution. We
find that each haem is bound in a pocket formed by the interface between a pair
of symmetry-related subunits. The quasi-twofold axis of the haem is closely
aligned with the local twofold axis relating these subunits. The axial ligands
of the haem are sulphurs of two equivalent methionyl residues (Met 52) from the
symmetry-related subunits. A cluster of four water molecules is trapped in the
gap between the upper edge of the haem and two extended protein loops which
close off the haem from the outer aqueous environment. This is the first
structure of a bis-methionine ligated haem-binding site and the first case of a
twofold symmetric haem-binding site.
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Secondary reference #1
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Title
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Location of haem in bacterioferritin of escherichia coli
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Authors
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F.Frolow,
A.J.Kalb(gilboa),
J.Yariv.
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Ref.
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acta crystallogr ,sect d, 1993,
49,
597.
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Secondary reference #2
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Title
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Amino acid sequence of the bacterioferritin (cytochrome b1) of escherichia coli-K12.
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Authors
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S.C.Andrews,
J.M.Smith,
J.R.Guest,
P.M.Harrison.
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Ref.
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Biochem Biophys Res Commun, 1989,
158,
489-496.
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PubMed id
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Secondary reference #3
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Title
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Molecular size and symmetry of the bacterioferritin of escherichia coli. X-Ray crystallographic characterization of four crystal forms.
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Authors
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J.M.Smith,
G.C.Ford,
P.M.Harrison,
J.Yariv,
A.J.Kalb.
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Ref.
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J Mol Biol, 1989,
205,
465-467.
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PubMed id
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Secondary reference #4
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Title
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The identity of bacterioferritin and cytochrome b1.
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Author
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J.Yariv.
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Ref.
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Biochem J, 1983,
211,
527.
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PubMed id
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Secondary reference #5
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Title
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The composition and the structure of bacterioferritin of escherichia coli.
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Authors
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J.Yariv,
A.J.Kalb,
R.Sperling,
E.R.Bauminger,
S.G.Cohen,
S.Ofer.
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Ref.
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Biochem J, 1981,
197,
171-175.
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PubMed id
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