The enzyme catalyzes the transfer of a phosphoryl group to a histidine residue of the CheY substrate protein. It uses a magnesium ion PMID11276258.
CATALYTIC METAL(S)
| Metal/Site Information | |||||
| Residue in MACiE* | Atom in MACiE* | Residue Type in MACiE | Physiological Metal |
Site Type |
Function(s) |
| MG 382 A | MG | magnesium | magnesium | mononuclear | Coordinates substrate Increases electrophilicity Electrostatic stabiliser |
*It refers to the MACiE reference pdb: 1b39
| Metal/s Properties in Resting State | ||||||
| MG 382 A | Resting state enzyme (-) | |||||
| Oxidation State | 2 | |||||
| Coordination Geometry | unknownGeometry | |||||
| Coordination Number | unknown | |||||
| Notes | - | |||||
| References |
| -Bilwes AM, Quezada CM, Croal LR, Crane BR, Simon MI Nucleotide binding by the histidine kinase CheA. Nat Struct Biol. 2001 Apr;8(4):353-60.(MEDLINE:11276258) |
| -Levit MN, Liu Y, Stock JB Mechanism of CheA protein kinase activation in receptor signaling complexes. Biochemistry. 1999 May 18;38(20):6651-8.(MEDLINE:10350484) |