The enzyme catalyzes the conversion of the aldo-sugars D-xylose or D-glucose to the keto-sugars D-xylulose and D-fructose, respectively. It binds two metal ions.
CATALYTIC METAL(S)
| Metal/Site Information | |||||
| Residue in MACiE* | Atom in MACiE* | Residue Type in MACiE | Physiological Metal |
Site Type |
Function(s) |
| MN 398 A | MN | manganese | divalent cation | mononuclear | Increases acidity Increases nucleophilicity |
| MN 399 A | MN | manganese | divalent cation | mononuclear | Coordinates substrate Electrostatic stabiliser |
*It refers to the MACiE reference pdb: 1xld
| Metal/s Properties in Resting State | ||||||
| MN 398 A | Resting state enzyme (1xib) | |||||
| Oxidation State | 2 | |||||
| Coordination Geometry | octahedral | |||||
| Coordination Number | 6 | |||||
| Notes | - | |||||
| MN 399 A | Resting state enzyme (1xib) | |||||
| Oxidation State | 2 | |||||
| Coordination Geometry | octahedral | |||||
| Coordination Number | 6 | |||||
| Notes | - | |||||
| References |
| -Evans G, Bricogne G Triiodide derivatization and combinatorial counter-ion replacement: two methods for enhancing phasing signal using laboratory Cu Kalpha X-ray equipment. Acta Crystallogr D Biol Crystallogr. 2002 Jun;58(Pt 6 Pt 2):976-91. Epub 2002 May 29.(MEDLINE:12037300) |
| -Fenn TD, Ringe D, Petsko GA Xylose isomerase in substrate and inhibitor michaelis states: atomic resolution studies of a metal-mediated hydride shift. Biochemistry. 2004 Jun 1;43(21):6464-74.(MEDLINE:15157080) |
| -Carrell HL, Hoier H, Glusker JP Modes of binding substrates and their analogues to the enzyme D-xylose isomerase. Acta Crystallogr D Biol Crystallogr. 1994 Mar 1;50(Pt 2):113-23.(MEDLINE:15299449) |
| -Collyer CA, Henrick K, Blow DM Mechanism for aldose-ketose interconversion by D-xylose isomerase involving ring opening followed by a 1,2-hydride shift. J Mol Biol. 1990 Mar 5;212(1):211-35.(MEDLINE:2319597) |