The enzyme catalyzes the cyclization of farnesyl diphosphate to trichodiene. It binds three magnesium ions which are directly involved in the reaction mechanism (PMID11698643).
CATALYTIC METAL(S)
| Metal/Site Information | |||||
| Residue in MACiE* | Atom in MACiE* | Residue Type in MACiE | Physiological Metal |
Site Type |
Function(s) |
| MG 701 B | MG | magnesium | magnesium | trinuclear | Coordinates substrate Promotes heterolysis Electrostatic stabiliser |
| MG 702 B | MG | magnesium | magnesium | trinuclear | Coordinates substrate Promotes heterolysis Electrostatic stabiliser |
| MG 703 B | MG | magnesium | magnesium | trinuclear | Coordinates substrate Promotes heterolysis Electrostatic stabiliser |
*It refers to the MACiE reference pdb: 1jfg
| Metal/s Properties in Resting State | ||||||
| MG 701 B | Resting state enzyme (-) | |||||
| Oxidation State | 2 | |||||
| Coordination Geometry | unknownGeometry | |||||
| Coordination Number | unknown | |||||
| Notes | - | |||||
| MG 702 B | Resting state enzyme (-) | |||||
| Oxidation State | 2 | |||||
| Coordination Geometry | unknownGeometry | |||||
| Coordination Number | unknown | |||||
| Notes | - | |||||
| MG 703 B | Resting state enzyme (-) | |||||
| Oxidation State | 2 | |||||
| Coordination Geometry | unknownGeometry | |||||
| Coordination Number | unknown | |||||
| Notes | - | |||||
| References |
| -Cane DE, Christianson DW Role of arginine-304 in the diphosphate-triggered active site closure mechanism of trichodiene synthase. Biochemistry. 2005 Sep 27;44(38):12719-27.(MEDLINE:16171386) |