The enzyme catalyzes the esteriļ¬cation of an amino acid to the 39-terminal adenosine of a tRNA PMID9736621. The enzyme uses Mg-ATP to perform the catalysis PMID16427818.
DETAILS ON METAL
Not available |
|
| A structure of the enzyme binding Mg-ATP is unavailable. However, kinetic studies proposed the presence of three distinct Mg site on the protein PMID16427818. |
| Site type | Physiological Metal |
| mononuclear | magnesium |
| Metal in the Resting State | |||
| Oxidation State |
Coordination Geometry | Coordination Number | PDB |
| 2 | unknownGeometry | unknown | - |
| Metal in the reaction stages | ||||||
| Step | Metal function(s) | Initial-Final Oxid. states | Initial-Final Geometry | Initial-Final Coord. # | Initial PDB | Final PDB |
| Step 1 | Coordinates substrate Increases electrophilicity Increases acidity |
+2 - +2 |
unknownGeometry - unknownGeometry |
unknown - unknown | - | - |
| Step 2 | Coordinates substrate Electrostatic stabiliser |
+2 - +2 |
unknownGeometry - unknownGeometry |
unknown - unknown | - | - |
| Step 3 | Coordinates substrate Electrostatic stabiliser |
+2 - +2 |
unknownGeometry - unknownGeometry |
unknown - unknown | - | - |
* N.B. There may be steps that are not described in between two annotated steps. Consequently the product species of step n may differ from the reagent species of step n+1.