The enzyme catalyzes the convertion of deamindo-NAD+ into NAD+. It uses Mg2+ to perform the catalysis (PMID9753692).
DETAILS ON METAL
MG 5001 x |
|
| The magnesium ions are directly involved in the catalytic mechanism (substrate binding and activation, charge stabilization) (PMID12077433). |
| Site type | Physiological Metal |
| mononuclear | magnesium |
| Metal in the Resting State | |||
| Oxidation State |
Coordination Geometry | Coordination Number | PDB |
| 2 | unknownGeometry | unknown | - |
| Metal in the reaction stages | ||||||
| Step | Metal function(s) | Initial-Final Oxid. states | Initial-Final Geometry | Initial-Final Coord. # | Initial PDB | Final PDB |
| Step 1 | Coordinates substrate Increases electrophilicity Electrostatic stabiliser |
+2 - +2 |
octahedral - octahedral |
6 - 6 | - | 1kqp |
| Step 2 | Coordinates substrate Electrostatic stabiliser |
+2 - +2 |
octahedral - octahedral |
6 - 6 | 1kqp | - |
| Step 3 | Coordinates substrate Electrostatic stabiliser |
+2 - +2 |
octahedral - octahedral |
6 - 6 | - | 2nsy |
* N.B. There may be steps that are not described in between two annotated steps. Consequently the product species of step n may differ from the reagent species of step n+1.