The enzyme catalyzes the convertion of deamindo-NAD+ into NAD+. It uses Mg2+ to perform the catalysis (PMID9753692).
CATALYTIC METAL(S)
| Metal/Site Information | |||||
| Residue in MACiE* | Atom in MACiE* | Residue Type in MACiE | Physiological Metal |
Site Type |
Function(s) |
| MG 5001 x | MG | magnesium | magnesium | mononuclear | Coordinates substrate Increases electrophilicity Electrostatic stabiliser |
| MG 5002 x | MG | magnesium | magnesium | mononuclear | Coordinates substrate Increases electrophilicity Electrostatic stabiliser |
*It refers to the MACiE reference pdb: 1kqp
| Metal/s Properties in Resting State | ||||||
| MG 5001 x | Resting state enzyme (-) | |||||
| Oxidation State | 2 | |||||
| Coordination Geometry | unknownGeometry | |||||
| Coordination Number | unknown | |||||
| Notes | - | |||||
| MG 5002 x | Resting state enzyme (-) | |||||
| Oxidation State | 2 | |||||
| Coordination Geometry | unknownGeometry | |||||
| Coordination Number | unknown | |||||
| Notes | - | |||||
| References |
| -Symersky J, Devedjiev Y, Moore K, Brouillette C, DeLucas L NH3-dependent NAD+ synthetase from Bacillus subtilis at 1 A resolution. Acta Crystallogr D Biol Crystallogr. 2002 Jul;58(Pt 7):1138-46. Epub 2002 Jun 20.(MEDLINE:12077433) |
| -Devedjiev Y, Symersky J, Singh R, Jedrzejas M, Brouillette C, Brouillette W, Muccio D, Chattopadhyay D, DeLucas L Stabilization of active-site loops in NH3-dependent NAD+ synthetase from Bacillus subtilis. Acta Crystallogr D Biol Crystallogr. 2001 Jun;57(Pt 6):806-12. Epub 2001 May 25.(MEDLINE:11375500) |