The enzyme catalyzes the hydroxylation of hypoxanthine to xanthine as well as xanthine to uric acid. It is an homodimeric enzyme and each subunit contains one molybdenum center, one flavin (FAD), and two [2Fe–2S] iron-sulfur centers (PMID15148401).
CATALYTIC METAL(S)
| Metal/Site Information | |||||
| Residue in MACiE* | Atom in MACiE* | Residue Type in MACiE | Physiological Metal |
Site Type |
Function(s) |
| FES 3001 A | FE1,FE2 | iron | iron | Fe2S2 | Electron relay |
| FES 3002 A | FE1,FE2 | iron | iron | Fe2S2 | Electron relay |
| MOS 3004 x | MO | molybdenum | molybdenum | molybdopterin | Coordinates substrate Electron pair acceptor One electron donor Increases nucleophilicity |
*It refers to the MACiE reference pdb: 1v97
| Metal/s Properties in Resting State | ||||||
| FES 3001 A | Resting state enzyme (-) | |||||
| Oxidation State | 2 | |||||
| Coordination Geometry | tetrahedral | |||||
| Coordination Number | 4 | |||||
| Notes | - | |||||
| FES 3002 A | Resting state enzyme (-) | |||||
| Oxidation State | 2 | |||||
| Coordination Geometry | tetrahedral | |||||
| Coordination Number | 4 | |||||
| Notes | - | |||||
| MOS 3004 x | Resting state enzyme (-) | |||||
| Oxidation State | 6 | |||||
| Coordination Geometry | squarePyramidal | |||||
| Coordination Number | 5 | |||||
| Notes | - | |||||
| References |
| -Okamoto K, Matsumoto K, Hille R, Eger BT, Pai EF, Nishino T The crystal structure of xanthine oxidoreductase during catalysis: implications for reaction mechanism and enzyme inhibition. Proc Natl Acad Sci U S A. 2004 May 25;101(21):7931-6. Epub 2004 May 17.(MEDLINE:15148401) |