The enzyme catalyzes the formation of two molecules of l-glutamate from 2-oxoglutarate and l-glutamine via intramolecular channeling of ammonia. It uses one FMN cofactor and one 3Fe-4S cluster as prosthetic groups (PMID11967268).
CATALYTIC METAL(S)
| Metal/Site Information | |||||
| Residue in MACiE* | Atom in MACiE* | Residue Type in MACiE | Physiological Metal |
Site Type |
Function(s) |
| F3S 2509 A | FE1,FE2,FE3 | iron | iron | Fe3S4 | Electron relay |
*It refers to the MACiE reference pdb: 1ofd
| Metal/s Properties in Resting State | ||||||
| F3S 2509 A | Resting state enzyme (1lm1) | |||||
| Oxidation State | 1 | |||||
| Coordination Geometry | tetrahedral | |||||
| Coordination Number | 4 | |||||
| Notes | The coordination number and the geometry refer to each single iron in the iron-sulfur cluster whereas the oxidation state is the formal oxidation state of the whole cluster. | |||||
| N.B.The coordination number and the geometry refer to each single iron in the iron-sulfur cluster whereas the oxidation state is the formal oxidation state of the whole cluster. | ||||||
| References |
| -van den Heuvel RH, Curti B, Vanoni MA, Mattevi A Glutamate synthase: a fascinating pathway from L-glutamine to L-glutamate. Cell Mol Life Sci. 2004 Mar;61(6):669-81.(MEDLINE:15052410) |
| -van den Heuvel RH, Ferrari D, Bossi RT, Ravasio S, Curti B, Vanoni MA, Florencio FJ, Mattevi A Structural studies on the synchronization of catalytic centers in glutamate synthase. J Biol Chem. 2002 Jul 5;277(27):24579-83. Epub 2002 Apr 19.(MEDLINE:11967268) |