The enzyme catalyzes the hydratation of nitriles to their corresponding amides. It contains iron (Fe3+) or cobalt (Co2+) as catalytic metal ions(PMID14717710).
CATALYTIC METAL(S)
| Metal/Site Information | |||||
| Residue in MACiE* | Atom in MACiE* | Residue Type in MACiE | Physiological Metal |
Site Type |
Function(s) |
| FE 300 A | FE | iron | cobalt/iron | mononuclear | Increases nucleophilicity Coordinates substrate |
*It refers to the MACiE reference pdb: 2ahj
| Metal/s Properties in Resting State | ||||||
| FE 300 A | Resting state enzyme (1ahj) | |||||
| Oxidation State | 3 | |||||
| Coordination Geometry | octahedral | |||||
| Coordination Number | 6 | |||||
| Notes | The sixth ligand is probably a water molecule or NO. | |||||
| References |
| -Kobayashi M, Shimizu S Nitrile hydrolases. Curr Opin Chem Biol. 2000 Feb;4(1):95-102.(MEDLINE:10679370) |