The enzyme catalyzes the hydrolysis of the C-N bond in beta-lactam compounds determining the inactivation of such antibiotics. Members of the beta-lactamase family have been grouped into four classes (A, B, C, D). Class B members employ either one (see M0016 entry) or two zinc ions in a dinuclear site (see M0015 entry) to effect beta-lactam cleavage (PMID10508665).
CATALYTIC METAL(S)
| Metal/Site Information | |||||
| Residue in MACiE* | Atom in MACiE* | Residue Type in MACiE | Physiological Metal |
Site Type |
Function(s) |
| ZN 1 A | ZN | zinc | zinc | dinuclear | Coordinates substrate Increases nucleophilicity Electrostatic stabiliser Increases acidity |
| ZN 2 A | ZN | zinc | zinc | dinuclear | Coordinates substrate Increases nucleophilicity Increases electrophilicity Increases acidity Electrostatic stabiliser |
*It refers to the MACiE reference pdb: 1znb
| Metal/s Properties in Resting State | ||||||
| ZN 1 A | Resting state enzyme (1znb) | |||||
| Oxidation State | 2 | |||||
| Coordination Geometry | tetrahedral | |||||
| Coordination Number | 4 | |||||
| Notes | - | |||||
| ZN 2 A | Resting state enzyme (1znb) | |||||
| Oxidation State | 2 | |||||
| Coordination Geometry | trigonalBipyramidal | |||||
| Coordination Number | 5 | |||||
| Notes | - | |||||
| References |
| -Spencer J, Read J, Sessions RB, Howell S, Blackburn GM, Gamblin SJ Antibiotic recognition by binuclear metallo-beta-lactamases revealed by X-ray crystallography. J Am Chem Soc. 2005 Oct 19;127(41):14439-44.(MEDLINE:16218639) |
| - Some information have been also deduced from the MACiE mechanism model |