PDBsum entry 9mux

Ligase

PDB id: 9mux

Contents

Protein chain

77 a.a.

Ligands

ARG-TRP-ALA-NH2

Metals

_ZN ×3

Waters ×81

PDB id:

9mux

Name:

Ligase

Title:

Structure of ubr1-rwa complex

Structure:

E3 ubiquitin-protein ligase ubr1. Chain: a. Fragment: ubr-box domain (unp residues 97-168). Synonym: n-recognin-1,ubiquitin-protein ligase e3-alpha-1,ubiquitin- protein ligase e3-alpha-i. Engineered: yes. Arg-trp-ala-nh2 peptide. Chain: d. Engineered: yes

Source:

Homo sapiens. Human. Organism_taxid: 9606. Gene: ubr1. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008. Synthetic: yes. Synthetic construct. Organism_taxid: 32630

Resolution:

1.29Å R-factor: 0.142 R-free: 0.151

Authors:

S.Huang,J.Wu,S.Taylor,Y.Chen

Key ref:

S.Huang et al. Structural flexibility and selectivity of n-End rule. To be published, .

Date:

14-Jan-25 Release date: 13-Aug-25

Protein chain

Q8IWV7  (UBR1_HUMAN) -  E3 ubiquitin-protein ligase UBR1 from Homo sapiens

Seq: 1749 a.a.

Struc: 77 a.a.*

* PDB and UniProt seqs differ at 5 residue positions (black crosses)

Enzyme reactions

• Enzyme class: E.C.2.3.2.27 - RING-type E3 ubiquitin transferase.
• Reaction: S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N₆- ubiquitinyl-[acceptor protein]-L-lysine