PDBsum entry 9h2s

Membrane protein

PDB id: 9h2s

Contents

Protein chains

(+ 1 more) 327 a.a.

Ligands

D12 ×21

PDB id:

9h2s

Name:

Membrane protein

Title:

A ynai-mscs chimera in a closed conformation purified in ddm with additional lipids showing ligand-filled pore and pockets

Structure:

Low conductance mechanosensitive channel ynai,small- conductance mechanosensitive channel. Chain: a, d, b, c, e, f, g. Engineered: yes

Source:

Escherichia coli (strain k12). Organism_taxid: 83333. Gene: ynai, b1330, jw1323, mscs, yggb, b2924, jw2891. Expressed in: escherichia coli. Expression_system_taxid: 562.

Authors:

V.J.Flegler,B.Bottcher,T.Rasmussen,A.Rasmussen,R.Hedrich

Key ref:

V.J.Flegler et al. (2025). Mechanosensitive channel engineering: a study on the and matching of ynai and mscs sensor paddles and pore. Nat commun, 16, 7881. PubMed id: 40849500 DOI: 10.1038/S41467-025-63253-0

Date:

15-Oct-24 Release date: 24-Sep-25

Protein chains

P0AEB5  (YNAI_ECOLI) -  Low conductance mechanosensitive channel YnaI from Escherichia coli (strain K12)

Seq: 343 a.a.

Struc: 327 a.a.*

Protein chains

P0C0S1  (MSCS_ECOLI) -  Small-conductance mechanosensitive channel from Escherichia coli (strain K12)

Seq: 286 a.a.

Struc: 327 a.a.*

* PDB and UniProt seqs differ at 223 residue positions (black crosses)