PDBsum entry 9fnn

Membrane protein

PDB id: 9fnn

Contents

Protein chains

793 a.a.

45 a.a.

485 a.a.

47 a.a.

240 a.a.

29 a.a.

50 a.a.

511 a.a.

41 a.a.

42 a.a.

144 a.a.

Ligands

C2E ×6

ATP ×2

Metals

_MG ×2

PDB id:

9fnn

Name:

Membrane protein

Title:

Cryo-em structure of thE C-di-gmp-saturated 'crown'less bcs macrocomplex for cellulose secretion in e. Coli

Structure:

Cellulose synthase catalytic subunit [udp-forming]. Chain: a. Engineered: yes. Mutation: yes. Other_details: purified bcs macrocomplex with stoichiometry bcsa- bcsb6-bcsr2-bcsq2-bcse2-bcsf2-bcsg3. Cyclic di-gmp-binding protein. Chain: b, x, y, z. Synonym: cellulose synthase regulatory subunit.

Source:

Escherichia coli. Organism_taxid: 562. Strain: 1094. Gene: bcsa, yhjo, yhjp, b3533, jw5665. Expressed in: escherichia coli. Expression_system_taxid: 562. Gene: bcsb, a8c65_00280, ac789_1c39010, acu57_05345, am464_10380, bhs81_21120, bk292_24560, bon72_13325, bon75_10020, bon76_21165, bon94_21155, bzl31_14275, c5p01_24165, c9162_26260, ci641_010915,

Authors:

I.Anso,P.V.Krasteva

Key ref:

I.Anso et al. Structural basis for synthase activation and cellulos modification in the e. Coli type ii bcs secretion sys. Nat commun, . PubMed id: 39394223

Date:

10-Jun-24 Release date: 16-Oct-24

Protein chain

P37653  (BCSA_ECOLI) -  Cellulose synthase catalytic subunit [UDP-forming] from Escherichia coli (strain K12)

Seq: 872 a.a.

Struc: 793 a.a.

Protein chain

P37652  (BCSB_ECOLI) -  Cyclic di-GMP-binding protein from Escherichia coli (strain K12)

Seq: 779 a.a.

Struc: 45 a.a.*

Protein chain

P37657  (BCSE_ECOLI) -  Cyclic di-GMP binding protein BcsE from Escherichia coli (strain K12)

Seq: 523 a.a.

Struc: 485 a.a.

Protein chain

P0ADJ5  (BCSF_ECOLI) -  Cellulose biosynthesis protein BcsF from Escherichia coli (strain K12)

Seq: 63 a.a.

Struc: 47 a.a.

Protein chains

P0DP92  (BCSQ_ECOLI) -  Cellulose biosynthesis protein BcsQ from Escherichia coli (strain K12)

Seq: 250 a.a.

Struc: 240 a.a.*

Protein chain

P0ADJ3  (YHJR_ECOLI) -  Protein YhjR from Escherichia coli (strain K12)

Seq: 62 a.a.

Struc: 29 a.a.

Protein chain

P0ADJ5  (BCSF_ECOLI) -  Cellulose biosynthesis protein BcsF from Escherichia coli (strain K12)

Seq: 63 a.a.

Struc: 50 a.a.

Protein chain

P37657  (BCSE_ECOLI) -  Cyclic di-GMP binding protein BcsE from Escherichia coli (strain K12)

Seq: 523 a.a.

Struc: 511 a.a.

Protein chain

P0ADJ3  (YHJR_ECOLI) -  Protein YhjR from Escherichia coli (strain K12)

Seq: 62 a.a.

Struc: 41 a.a.

Protein chains

P37652  (BCSB_ECOLI) -  Cyclic di-GMP-binding protein from Escherichia coli (strain K12)

Seq: 779 a.a.

Struc: 42 a.a.*

Protein chains

P37659  (BCSG_ECOLI) -  Cellulose biosynthesis protein BcsG from Escherichia coli (strain K12)

Seq: 559 a.a.

Struc: 144 a.a.

* PDB and UniProt seqs differ at 3 residue positions (black crosses)

Enzyme reactions

• Enzyme class: Chain A: E.C.2.4.1.12 - cellulose synthase (UDP-forming).
• Reaction: [(1->4)-beta-D-glucosyl](n) + UDP-alpha-D-glucose = [(1->4)-beta-D- glucosyl](n+1) + UDP + H⁺

[(1->4)-beta-D-glucosyl](n) + UDP-alpha-D-glucose = [(1->4)-beta-D- glucosyl](n+1) + UDP + H(+) (Bound ligand (Het Group name = ATP) matches with 69.70% similarity)

Molecule diagrams generated from .mol files obtained from the KEGG ftp site