PDBsum entry 9ddr

Replication

PDB id: 9ddr

Contents

Protein chain

367 a.a.

DNA/RNA

Ligands

TTP

Metals

_CA ×2

Waters ×144

PDB id:

9ddr

Name:

Replication

Title:

Ternary substrate complex of DNA polymerase iota with DNA (template a), ca2+, and dttp

Structure:

DNA polymerase iota. Chain: a. Synonym: eta2,rad30 homolog b. Engineered: yes. Other_details: polymerase iota catalytic core. DNA (5'- d(p Ap Ap Gp Gp Gp Tp Cp Cp Tp Ap Gp Gp Ap Cp Cp C)-3'). Chain: b. Engineered: yes.

Source:

Homo sapiens. Human. Organism_taxid: 9606. Gene: poli, rad30b. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008. Synthetic: yes. Organism_taxid: 9606

Resolution:

2.15Å R-factor: 0.239 R-free: 0.280

Authors:

Z.Frevert,D.Reusch,B.Freudenthal,M.T.Washington

Key ref:

Z.Frevert et al. (2025). Visualizing DNA polymerase iota catalyze hoogsteen-Di DNA synthesis. Nat commun, 16, 5979. PubMed id: 40593703

Date:

28-Aug-24 Release date: 09-Jul-25

Protein chain

Q9UNA4  (POLI_HUMAN) -  DNA polymerase iota from Homo sapiens

Seq: 740 a.a.

Struc: 367 a.a.

DNA/RNA chain

A-A-G-G-G-T-C-C-T-A-G-G-A-C-C-C 16 bases

Enzyme reactions

• Enzyme class: E.C.2.7.7.7 - DNA-directed Dna polymerase.
• Reaction: DNA(n) + a 2'-deoxyribonucleoside 5'-triphosphate = DNA(n+1) + diphosphate

Molecule diagrams generated from .mol files obtained from the KEGG ftp site