PDBsum entry 8yii

Structural protein/RNA

PDB id: 8yii

Contents

Protein chains

1541 a.a.

16 a.a.

DNA/RNA

Ligands

ADP

Metals

_MG ×5

PDB id:

8yii

Name:

Structural protein/RNA

Title:

Dmdcr-2/loqspd/slm2 in dicing state

Structure:

Dicer-2, isoform a. Chain: a. Synonym: fi15132p1. Ec: 3.1.21.1,3.1.26.-,3.1.26.3,3.6.1.3. Engineered: yes. Isoform pd of protein loquacious. Chain: b. Engineered: yes. Slm2.

Source:

Drosophila melanogaster. Fruit fly. Organism_taxid: 7227. Gene: dcr-2, cg6493, dcr, dcr, dcr-2, dcr-2, dcr-2-ra, dcr2, dcr2, dcr2, ddcr2, dic2, dicer, dicer, dicer, dicer-2, dicer-2, dicer2, dicer2, dmdcr-2, dmel\cg6493, cg6493, dmel_cg6493. Expressed in: spodoptera frugiperda. Expression_system_taxid: 7108. Gene: loqs, drax, loq, r3d1, trbp, cg6866.

Authors:

N.Cao,S.Su,J.Wang,J.Ma,H.-W.Wang

Key ref:

N.Cao et al. (2025). Structural basis of endo-Sirna processing by drosophi dicer-2 and loqs-Pd.. Nucleic acids res, 53, . PubMed id: 39988314

Date:

29-Feb-24 Release date: 05-Mar-25

Protein chain

A1ZAW0  (A1ZAW0_DROME) -  Endoribonuclease Dcr-2 from Drosophila melanogaster

Seq: 1722 a.a.

Struc: 1541 a.a.*

Protein chain

Q9VJY9  (Q9VJY9_DROME) -  Protein Loquacious from Drosophila melanogaster

Seq: 465 a.a.

Struc: 16 a.a.*

* PDB and UniProt seqs differ at 16 residue positions (black crosses)

DNA/RNA chain

G-G-U-U-C-G-C-U-C-C-C-G-G-C-G-C-U-U-C-A-C-A-G-G-C-G-C-U-G-G-A-A-A-A-U-C-U-U-A- 104 bases

Enzyme reactions

• Enzyme class: Chain A: E.C.3.1.26.3 - ribonuclease Iii.
• Reaction: Endonucleolytic cleavage to 5'-phosphomonoester.