PDBsum entry 8xn4

Hydrolase

PDB id: 8xn4

Contents

Protein chains

(+ 1 more) 182 a.a.

(+ 1 more) 176 a.a.

PDB id:

8xn4

Name:

Hydrolase

Title:

Cryo-em structure of the clpp degradation system in streptomyces hawaiiensis

Structure:

Atp-dependent clp protease proteolytic subunit. Chain: a, b, c, d, e, f, g. Synonym: endopeptidase clp. Engineered: yes. Mutation: yes. Atp-dependent clp protease proteolytic subunit. Chain: h, i, j, k, l, m, n. Synonym: endopeptidase clp. Engineered: yes.

Source:

Streptomyces hawaiiensis. Organism_taxid: 67305. Gene: clpp1, clpp, ceb94_14110. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008. Gene: clpp2, clpp, ceb94_14105. Expression_system_taxid: 469008

Authors:

X.Xu,F.Long

Key ref:

X.Xu et al. Structural insights into the clp protein degradation machinery.. Mbio, . PubMed id: 38501868

Date:

29-Dec-23 Release date: 27-Mar-24

Protein chains

A0A5B9BGY8  (A0A5B9BGY8_9ACTN) -  ATP-dependent Clp protease proteolytic subunit from Streptomyces hawaiiensis

Seq: 219 a.a.

Struc: 182 a.a.*

Protein chains

A0A5B9BIX9  (A0A5B9BIX9_9ACTN) -  ATP-dependent Clp protease proteolytic subunit from Streptomyces hawaiiensis

Seq: 235 a.a.

Struc: 176 a.a.*

* PDB and UniProt seqs differ at 2 residue positions (black crosses)

Enzyme reactions

• Enzyme class: Chains A, B, C, D, E, F, G, H, I, J, K, L, M, N: E.C.3.4.21.92 - endopeptidase Clp.
• Reaction: Hydrolysis of proteins to small peptides in the presence of ATP and magnesium. Alpha-casein is the usual test substrate. In the absence of ATP, only oligopeptides shorter than five residues are cleaved (such as succinyl-Leu-Tyr-|-NHMEC; and Leu-Tyr-Leu-|-Tyr-Trp, in which the cleavage of the -Tyr-|-Leu- and -Tyr-|-Trp- bond also occurs).