PDBsum entry 8vms

Hydrolase

PDB id: 8vms

Contents

Protein chains

162 a.a.

DNA/RNA

Ligands

GOL ×5

Metals

_NA ×2

_MG ×2

_ZN ×4

Waters ×402

PDB id:

8vms

Name:

Hydrolase

Title:

Homing endonuclease i-ppoi-DNA complex:reaction at ph7.0 (k+ mes) with 500 um mg2+ for 80s

Structure:

DNA (5'- d( Tp Tp Gp Ap Cp Tp Cp Tp Cp Tp Tp Ap Ap Gp Ap Gp Ap Gp Tp Cp A)- 3'). Chain: c, d. Engineered: yes. Intron-encoded endonuclease i-ppoi. Chain: a, b. Synonym: i-ppo. Engineered: yes

Source:

Synthetic: yes. Synthetic construct. Organism_taxid: 32630. Physarum polycephalum. Slime mold. Organism_taxid: 5791. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008

Resolution:

1.42Å R-factor: 0.180 R-free: 0.189

Authors:

C.Chang,Y.Gao

Key ref:

C.Chang and y.gao Homing endonuclease i-Ppoi-Dna complex:reaction with mg2+ for 160s. To be published, . PubMed id: 39141555

Date:

13-Jan-24 Release date: 10-Jul-24

Protein chains

Q94702  (PPO1_PHYPO) -  Intron-encoded endonuclease I-PpoI from Physarum polycephalum

Seq: 163 a.a.

Struc: 162 a.a.

DNA/RNA chains

T-T-G-A-C-T-C-T-C-T-T-A-A-G-A-G-A-G-T-C-A 21 bases

T-T-G-A-C-T-C-T-C-T-T-A-A-G-A-G-A-G-T-C-A 21 bases

Enzyme reactions

• Enzyme class: E.C.3.1.-.-