PDBsum entry 8ubc

RNA binding protein/RNA

PDB id: 8ubc

Contents

Protein chains

315 a.a.

122 a.a.

111 a.a.

DNA/RNA

PDB id:

8ubc

Name:

RNA binding protein/RNA

Title:

Diversity-generating retroelement (dgr) ribonucleoprotein reverse transcriptase - resting state 1b

Structure:

Reverse transcriptase. Chain: a. Engineered: yes. Avd. Chain: b, c, d, e, f. Synonym: formaldehyde-activating enzyme,fae. Engineered: yes. Diversity-generating retroelement (dgr) RNA tr. Chain: h.

Source:

Bordetella phage bpp-1. Organism_taxid: 2885909. Gene: brt. Expressed in: escherichia coli. Expression_system_taxid: 562. Gene: bbp7, fae, mexam1_meta1p1766. Synthetic: yes. Organism_taxid: 2885909

Authors:

T.Biswas,S.Handa,P.Ghosh

Key ref:

S.Handa et al. Rna control of reverse transcription in a diversity-Generating retroelement. Nature, . PubMed id: 39779855

Date:

22-Sep-23 Release date: 23-Oct-24

Protein chain

Q775D8  (Q775D8_BPBPP) -  Reverse transcriptase from Bordetella phage BPP-1

Seq: 328 a.a.

Struc: 315 a.a.

Protein chain

Q775D7  (Q775D7_BPBPP) -  Bbp7 from Bordetella phage BPP-1

Seq: 128 a.a.

Struc: 122 a.a.

Protein chain

Q9FA38  (FAE_METEA) -  5,6,7,8-tetrahydromethanopterin hydro-lyase from Methylorubrum extorquens (strain ATCC 14718 / DSM 1338 / JCM 2805 / NCIMB 9133 / AM1)

Seq: 170 a.a.

Struc: 122 a.a.*

Protein chains

Q775D7  (Q775D7_BPBPP) -  Bbp7 from Bordetella phage BPP-1

Seq: 128 a.a.

Struc: 111 a.a.

Protein chains

Q9FA38  (FAE_METEA) -  5,6,7,8-tetrahydromethanopterin hydro-lyase from Methylorubrum extorquens (strain ATCC 14718 / DSM 1338 / JCM 2805 / NCIMB 9133 / AM1)

Seq: 170 a.a.

Struc: 111 a.a.*

* PDB and UniProt seqs differ at 208 residue positions (black crosses)

DNA/RNA chains

A-U-C-A-C-C-U 7 bases

U-G-G-C-U-C-U-G-C-C-A-A-C-G-C-U-A-C-G-G-C-U-U-G-G-C-G-G-G-C-U-U-A-G-G-U-G-G-G- 86 bases

Enzyme reactions

• Enzyme class: Chains B, C, D, E, F: E.C.4.2.1.147 - 5,6,7,8-tetrahydromethanopterin hydro-lyase.
• Reaction: 5,6,7,8-tetrahydromethanopterin + formaldehyde = 5,10- methylenetetrahydromethanopterin + H2O

Molecule diagrams generated from .mol files obtained from the KEGG ftp site