PDBsum entry 8sso

Transferase

PDB id: 8sso

Contents

Protein chains

256 a.a.

92 a.a.

86 a.a.

Ligands

EDO

ACT ×2

627 ×2

Waters ×536

PDB id:

8sso

Name:

Transferase

Title:

Aura bound to danusertib and inhibiting monobody mb2

Structure:

Aurora kinase a. Chain: a, d. Synonym: aurora 2,aurora/ipl1-related kinase 1,ark-1,aurora-related kinase 1,breast tumor-amplified kinase,ipl1- and aurora-related kinase 1,serine/threonine-protein kinase 15,serine/threonine-protein kinase 6,serine/threonine-protein kinase ayk1,serine/threonine- protein kinase aurora-a. Engineered: yes. Mb2.

Source:

Homo sapiens. Human. Organism_taxid: 9606. Gene: aurka, aik, airk1, ark1, aura, ayk1, btak, iak1, stk15, stk6. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008. Synthetic construct. Organism_taxid: 32630. Expression_system_taxid: 469008

Resolution:

1.97Å R-factor: 0.194 R-free: 0.234

Authors:

H.Ludewig,C.Kim,D.Kern

Key ref:

C.Kim et al. (2023). A biophysical framework for double-Drugging kinases.. Proc.Natl.Acad.Sci.Usa, 120, 11120. PubMed id: 37590418 DOI: 10.1073/PNAS.2304611120

Date:

08-May-23 Release date: 06-Sep-23

Protein chains

O14965  (AURKA_HUMAN) -  Aurora kinase A from Homo sapiens

Seq: 403 a.a.

Struc: 256 a.a.

Protein chain

No UniProt id for this chain

Struc: 92 a.a.

Protein chain

No UniProt id for this chain

Struc: 86 a.a.

Enzyme reactions

• Enzyme class: Chains A, D: E.C.2.7.11.1 - non-specific serine/threonine protein kinase.
• Reaction:
  1. L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H⁺
  2. L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H⁺

Molecule diagrams generated from .mol files obtained from the KEGG ftp site