PDBsum entry 8sdi

Ligase

PDB id: 8sdi

Contents

Protein chains

80 a.a.

PDB id:

8sdi

Name:

Ligase

Title:

Hydrophobic interactions drive the tetrameric assembly of the trim45 coiled-coil domain

Structure:

Tripartite motif-containing protein 45. Chain: a, b, c, d. Fragment: coiled coil domain. Engineered: yes

Source:

Mus musculus. House mouse. Organism_taxid: 10090. Gene: trim45. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008

Resolution:

2.70Å R-factor: 0.239 R-free: 0.271

Authors:

X.H.Lou,B.B.Ma,Y.Zhuang,X.C.Li

Key ref:

X.H.Lou et al. Hydrophobic interactions drive the tetrameric assembl the trim45 coiled-Coil domain. To be published, .

Date:

06-Apr-23 Release date: 24-Apr-24

Protein chains

Q6PFY8  (TRI45_MOUSE) -  E3 ubiquitin-protein ligase TRIM45 from Mus musculus

Seq: 580 a.a.

Struc: 80 a.a.*

* PDB and UniProt seqs differ at 1 residue position (black cross)

Enzyme reactions

• Enzyme class: E.C.2.3.2.27 - RING-type E3 ubiquitin transferase.
• Reaction: S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N₆- ubiquitinyl-[acceptor protein]-L-lysine