PDBsum entry 8s87

Transferase

PDB id: 8s87

Contents

Protein chain

753 a.a.

Ligands

SO4 ×5

GOL

Metals

_MG ×9

_NA ×2

Waters ×153

PDB id:

8s87

Name:

Transferase

Title:

Kod-h4 DNA polymerase mutant - apo structure

Structure:

DNA polymerase. Chain: a. Engineered: yes

Source:

Thermococcus kodakarensis kod1. Organism_taxid: 69014. Expressed in: escherichia coli. Expression_system_taxid: 562

Resolution:

1.82Å R-factor: 0.231 R-free: 0.271

Authors:

C.Gutfreund,K.Betz

Key ref:

C.Gutfreund et al. Structural insights into a DNA polymerase reading the nucleic acid hna.. Nucleic acids res, . PubMed id: 39673482

Date:

06-Mar-24 Release date: 13-Nov-24

Protein chain

D0VWU9  (D0VWU9_THEKO) -  DNA polymerase from Thermococcus kodakarensis (strain ATCC BAA-918 / JCM 12380 / KOD1)

Seq: 774 a.a.

Struc: 753 a.a.*

* PDB and UniProt seqs differ at 19 residue positions (black crosses)

Enzyme reactions

• Enzyme class: E.C.2.7.7.7 - DNA-directed Dna polymerase.
• Reaction: DNA(n) + a 2'-deoxyribonucleoside 5'-triphosphate = DNA(n+1) + diphosphate

Molecule diagrams generated from .mol files obtained from the KEGG ftp site