PDBsum entry 8hx8

Transferase

PDB id: 8hx8

Contents

Protein chains

673 a.a.

Ligands

TRP ×2

SIN

Metals

_MG ×2

Waters ×2

PDB id:

8hx8

Name:

Transferase

Title:

Crystal structure of 4-amino-4-deoxychorismate synthase from streptomyces venezuelae co-crystallized with chorismate

Structure:

4-amino-4-deoxychorismate synthase. Chain: a, b. Synonym: aminodeoxychorismate synthase. Engineered: yes

Source:

Streptomyces venezuelae. Organism_taxid: 54571. Gene: papa. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008.

Resolution:

2.55Å R-factor: 0.209 R-free: 0.268

Authors:

Y.Nakamichi,M.Watanabe

Key ref:

Y.Nakamichi et al. (2023). Structural basis for the allosteric pathway of 4-Amino-4-Deoxychorismate synthase.. Acta crystallogr d struct, 79, 895. PubMed id: 37712435

Date:

04-Jan-23 Release date: 18-Oct-23

Protein chains

Q6L8Q5  (Q6L8Q5_STRVZ) -  aminodeoxychorismate synthase from Streptomyces venezuelae

Seq: 686 a.a.

Struc: 673 a.a.*

* PDB and UniProt seqs differ at 1 residue position (black cross)

Enzyme reactions

• Enzyme class: E.C.2.6.1.85 - aminodeoxychorismate synthase.
• Reaction: chorismate + L-glutamine = 4-amino-4-deoxychorismate + L-glutamate

chorismate + L-glutamine (Bound ligand (Het Group name = TRP) matches with 56.25% similarity) = 4-amino-4-deoxychorismate + L-glutamate

• Cofactor: Mg(2+)

Molecule diagrams generated from .mol files obtained from the KEGG ftp site