PDBsum entry 8hpc

Hydrolase

PDB id: 8hpc

Contents

Protein chains

(+ 2 more) 300 a.a.

Ligands

M9L ×8

Waters ×270

PDB id:

8hpc

Name:

Hydrolase

Title:

Crystal structure of c171a mutant of n-carbamyl-d-amino acid amidohydrolase complexed with n-carbamyl-d-hydroxyphenylglycine

Structure:

N-carbamoyl-d-amino acid hydrolase. Chain: a, b, c, d, e, f, g, h. Synonym: d-n-alpha-carbamilase. Engineered: yes. Mutation: yes

Source:

Agrobacterium sp. Knk712. Organism_taxid: 252128. Expressed in: escherichia coli. Expression_system_taxid: 562

Resolution:

2.52Å R-factor: 0.220 R-free: 0.253

Authors:

L.D.Zhang,W.Song

Key ref:

L.D.Zhang and w.song Production of d-P-Hydroxyphenylglycine by double-Enzy cascade. To be published, .

Date:

12-Dec-22 Release date: 20-Dec-23

Protein chains

P60327  (DCAS_AGRSK) -  N-carbamoyl-D-amino acid hydrolase from Agrobacterium sp. (strain KNK712)

Seq: 304 a.a.

Struc: 300 a.a.*

* PDB and UniProt seqs differ at 1 residue position (black cross)

Enzyme reactions

• Enzyme class: E.C.3.5.1.77 - N-carbamoyl-D-amino-acid hydrolase.
• Reaction: an N-carbamoyl-D-amino acid + H2O + 2 H⁺ = a D-alpha-amino acid + NH4⁺ + CO2

N-carbamoyl-D-amino acid (Bound ligand (Het Group name = M9L) matches with 50.00% similarity) + H2O + 2 × H(+) = D-alpha-amino acid + NH4(+) + CO2

Molecule diagrams generated from .mol files obtained from the KEGG ftp site