PDBsum entry 8hnt

Immune system

PDB id: 8hnt

Contents

Protein chains

1000 a.a.

88 a.a.

DNA/RNA

PDB id:

8hnt

Name:

Immune system

Title:

Crystal structure of anti-crispr protein acriic4 bound to hpacas9- sgrna surveillance complex

Structure:

Crispr-associated endonuclease cas9. Chain: a. Engineered: yes. Sgrna. Chain: b. Engineered: yes. Other_details: RNA is originally derived from haemophilus parainfluenzae and modified by author.. Anti-crispr protein acriic4.

Source:

Haemophilus parainfluenzae. Organism_taxid: 729. Gene: csn1. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008. Synthetic: yes. Synthetic construct. Organism_taxid: 32630. Gene: acriic4.

Resolution:

3.06Å R-factor: 0.238 R-free: 0.264

Authors:

W.Sun,Z.Cheng,Y.Wang

Key ref:

W.Sun et al. Structure of hpacas9-Sgrna-Dsdna in the presence of a. To be published, . PubMed id: 37494395

Date:

08-Dec-22 Release date: 19-Jul-23

Protein chain

F0ET08  (F0ET08_HAEPA) -  CRISPR-associated endonuclease Cas9 from Haemophilus parainfluenzae ATCC 33392

Seq: 1054 a.a.

Struc: 1000 a.a.

Protein chain

A0A377JKY9  (A0A377JKY9_HAEPA) -  Uncharacterized protein from Haemophilus parainfluenzae

Seq: 88 a.a.

Struc: 88 a.a.*

* PDB and UniProt seqs differ at 3 residue positions (black crosses)

DNA/RNA chain

U-C-A-C-U-C-U-U-A-A-U-C-A-C-A-C-G-U-U-G-U-A-G-C-U-C-C-C-U-U-U-A-C-G-U-U-G-U-U- 102 bases

Enzyme reactions

• Enzyme class 2: Chain A: E.C.3.1.-.-
• Enzyme class 3: Chain C: E.C.?

Note, where more than one E.C. class is given (as above), each may correspond to a different protein domain or, in the case of polyprotein precursors, to a different mature protein.