PDBsum entry 8hfb

Oxidoreductase

PDB id: 8hfb

Contents

Protein chains

319 a.a.

Ligands

EDO ×4

GOL

Metals

_NI ×4

Waters ×187

PDB id:

8hfb

Name:

Oxidoreductase

Title:

Evolved variant of quercetin 2,4-dioxygenase from bacillus subtilis

Structure:

Quercetin 2,3-dioxygenase. Chain: a, b. Synonym: quercetinase,flavonol 2,4-dioxygenase. Engineered: yes. Mutation: yes

Source:

Bacillus subtilis. Organism_taxid: 1423. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008

Resolution:

2.24Å R-factor: 0.215 R-free: 0.251

Authors:

H.Eom,W.J.Song

Key ref:

H.Eom et al. Underlying role of hydrophobic environments in tuning elements for efficient enzyme catalysis.. J am chem soc, . PubMed id: 36853654

Date:

10-Nov-22 Release date: 08-Mar-23

Protein chains

P42106  (QDOI_BACSU) -  Quercetin 2,3-dioxygenase from Bacillus subtilis (strain 168)

Seq: 337 a.a.

Struc: 319 a.a.*

* PDB and UniProt seqs differ at 7 residue positions (black crosses)

Enzyme reactions

• Enzyme class: E.C.1.13.11.24 - quercetin 2,3-dioxygenase.
• Pathway: Quercetin 2,3-Dioxygenase
• Reaction: quercetin + O2 = 2-(3,4-dihydroxybenzoyloxy)-4,6-dihydroxybenzoate + CO

quercetin + O2 = 2-(3,4-dihydroxybenzoyloxy)-4,6-dihydroxybenzoate + CO (Bound ligand (Het Group name = EDO) matches with 50.00% similarity)

• Cofactor: Fe cation or Cu cation

Molecule diagrams generated from .mol files obtained from the KEGG ftp site