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UniProt functional annotation for P42106 | ||
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| UniProt code: P42106. |
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| Organism: | |
Bacillus subtilis (strain 168). |
| Taxonomy: | |
Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus. |
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| Function: | |
Performs the first step in the degradation of the flavonoid quercetin by a dioxygenase reaction. The enzyme catalyzes the cleavage of the O-heteroaromatic ring of the flavonol quercetin yielding the depside 2-protocatechuoyl-phloroglucinol carboxylic acid and carbon monoxide. This involves the remarkable dioxygenolytic cleavage of two carbon-carbon bonds. {ECO:0000269|PubMed:14741339}. |
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| Catalytic activity: | |
Reaction=O2 + quercetin = 2-(3,4-dihydroxybenzoyloxy)-4,6- dihydroxybenzoate + CO; Xref=Rhea:RHEA:15381, ChEBI:CHEBI:15379, ChEBI:CHEBI:17245, ChEBI:CHEBI:57628, ChEBI:CHEBI:57694; EC=1.13.11.24; |
| Cofactor: | |
Name=Fe(2+); Xref=ChEBI:CHEBI:29033; Evidence={ECO:0000269|PubMed:15039076}; Note=Binds 2 Fe(2+) ions per subunit. {ECO:0000269|PubMed:15039076}; |
| Biophysicochemical properties: | |
Kinetic parameters: KM=3.8 uM for quercetin {ECO:0000269|PubMed:14741339}; Vmax=2 umol/min/mg enzyme {ECO:0000269|PubMed:14741339}; |
| Pathway: | |
Flavonoid metabolism; quercetin degradation. |
| Subunit: | |
Homodimer. |
| Mass spectrometry: | |
Mass=38658; Method=MALDI; Evidence={ECO:0000269|PubMed:14741339}; |
Annotations taken from UniProtKB at the EBI.