PDBsum entry 8hah

Transferase/DNA

PDB id: 8hah

Contents

Protein chains

103 a.a.

80 a.a.

107 a.a.

97 a.a.

517 a.a.

DNA/RNA

PDB id:

8hah

Name:

Transferase/DNA

Title:

Cryo-em structure of the p300 catalytic core bound to the h4k12ack16ac nucleosome, class 2 (3.9 angstrom resolution)

Structure:

Histone h3.1. Chain: a, e. Synonym: histone h3/a,histone h3/b,histone h3/c,histone h3/d,histone h3/f,histone h3/h,histone h3/i,histone h3/j,histone h3/k,histone h3/l. Engineered: yes. Histone h4. Chain: b, f. Engineered: yes.

Source:

Homo sapiens. Human. Organism_taxid: 9606. Gene: h3c1, h3fa, hist1h3a, h3c2, h3fl, hist1h3b, h3c3, h3fc hist1h3c, h3c4, h3fb, hist1h3d, h3c6, h3fd, hist1h3e, h3c7, h3fi, hist1h3f, h3c8, h3fh, hist1h3g, h3c10, h3fk, hist1h3h, h3c11, h3ff, hist1h3i, h3c12, h3fj, hist1h3j. Expressed in: escherichia coli. Expression_system_taxid: 562.

Authors:

M.Kikuchi,S.Morita,M.Wakamori,S.Shin,T.Uchikubo-Kamo,M.Shirouzu, T.Umehara

Key ref:

M.Kikuchi et al. Epigenetic mechanisms to propagate histone acetylatio p300/cbp. To be published, . PubMed id: 37460559

Date:

26-Oct-22 Release date: 17-May-23

Protein chains

P68431  (H31_HUMAN) -  Histone H3.1 from Homo sapiens

Seq: 136 a.a.

Struc: 103 a.a.

Protein chains

P62805  (H4_HUMAN) -  Histone H4 from Homo sapiens

Seq: 103 a.a.

Struc: 80 a.a.

Protein chains

P04908  (H2A1B_HUMAN) -  Histone H2A type 1-B/E from Homo sapiens

Seq: 130 a.a.

Struc: 107 a.a.

Protein chains

P06899  (H2B1J_HUMAN) -  Histone H2B type 1-J from Homo sapiens

Seq: 126 a.a.

Struc: 97 a.a.

Protein chain

Q09472  (EP300_HUMAN) -  Histone acetyltransferase p300 from Homo sapiens

Seq: 2414 a.a.

Struc: 517 a.a.

DNA/RNA chains

A-C-C-G-C-C-A-T-C-A-A-T-A-T-C-C-A-C-C-T-G-C-A-G-A-T-T-C-T-A-C-C-A-A-A-A-G-T-G- 164 bases

T-C-C-G-T-T-A-C-C-G-C-C-A-T-C-A-A-T-A-T-C-C-A-C-C-T-G-C-A-G-A-T-T-C-T-A-C-C-A- 164 bases

Enzyme reactions

• Enzyme class 1: Chain K: E.C.2.3.1.- - ?????
• Enzyme class 2: Chain K: E.C.2.3.1.48 - histone acetyltransferase.
• Reaction: L-lysyl-[protein] + acetyl-CoA = N₆-acetyl-L-lysyl-[protein] + CoA + H⁺

Note, where more than one E.C. class is given (as above), each may correspond to a different protein domain or, in the case of polyprotein precursors, to a different mature protein.

Molecule diagrams generated from .mol files obtained from the KEGG ftp site