PDBsum entry 8h3a

Ligase

PDB id: 8h3a

Contents

Protein chains

309 a.a.

412 a.a.

420 a.a.

407 a.a.

406 a.a.

288 a.a.

214 a.a.

177 a.a.

556 a.a.

718 a.a.

89 a.a.

Metals

_ZN ×4

PDB id:

8h3a

Name:

Ligase

Title:

Cryo-em structure of the kbtbd2-crl3~n8(removed)-csn complex

Structure:

Cop9 signalosome complex subunit 5. Chain: e. Engineered: yes. Cop9 signalosome complex subunit 1. Chain: a. Synonym: sgn1,signalosome subunit 1,g protein pathway suppressor 1, gps-1,jab1-containing signalosome subunit 1,protein mfh. Engineered: yes. Cop9 signalosome complex subunit 2.

Source:

Homo sapiens. Human. Organism_taxid: 9606. Gene: cops5, csn5, jab. Expressed in: trichoplusia ni. Expression_system_taxid: 7111. Gene: gps1, cops1, csn1. Gene: cops2, csn2, trip15. Gene: cops3, csn3.

Authors:

Y.Hu,Q.Mao,Z.Chen,L.Sun

Key ref:

Y.Hu et al. Cryo-Em structure of the kbtbd2-Crl3~n8-Csn complex. To be published, . PubMed id: 38332366

Date:

08-Oct-22 Release date: 11-Oct-23

Protein chain

Q92905  (CSN5_HUMAN) -  COP9 signalosome complex subunit 5 from Homo sapiens

Seq: 334 a.a.

Struc: 309 a.a.

Protein chain

Q13098  (CSN1_HUMAN) -  COP9 signalosome complex subunit 1 from Homo sapiens

Seq: 491 a.a.

Struc: 412 a.a.

Protein chain

P61201  (CSN2_HUMAN) -  COP9 signalosome complex subunit 2 from Homo sapiens

Seq: 443 a.a.

Struc: 420 a.a.

Protein chain

Q9UNS2  (CSN3_HUMAN) -  COP9 signalosome complex subunit 3 from Homo sapiens

Seq: 423 a.a.

Struc: 407 a.a.

Protein chain

Q9BT78  (CSN4_HUMAN) -  COP9 signalosome complex subunit 4 from Homo sapiens

Seq: 406 a.a.

Struc: 406 a.a.

Protein chain

Q7L5N1  (CSN6_HUMAN) -  COP9 signalosome complex subunit 6 from Homo sapiens

Seq: 327 a.a.

Struc: 288 a.a.

Protein chain

Q9H9Q2  (CSN7B_HUMAN) -  COP9 signalosome complex subunit 7b from Homo sapiens

Seq: 264 a.a.

Struc: 214 a.a.

Protein chain

Q99627  (CSN8_HUMAN) -  COP9 signalosome complex subunit 8 from Homo sapiens

Seq: 209 a.a.

Struc: 177 a.a.

Protein chains

Q8IY47  (KBTB2_HUMAN) -  Kelch repeat and BTB domain-containing protein 2 from Homo sapiens

Seq: 623 a.a.

Struc: 556 a.a.*

Protein chain

Q13618  (CUL3_HUMAN) -  Cullin-3 from Homo sapiens

Seq: 768 a.a.

Struc: 718 a.a.

Protein chain

P62877  (RBX1_HUMAN) -  E3 ubiquitin-protein ligase RBX1 from Homo sapiens

Seq: 108 a.a.

Struc: 89 a.a.

* PDB and UniProt seqs differ at 1 residue position (black cross)

Enzyme reactions

• Enzyme class 2: Chain E: E.C.3.4.-.-
• Enzyme class 3: Chain R: E.C.2.3.2.27 - RING-type E3 ubiquitin transferase.
• Reaction: S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N₆- ubiquitinyl-[acceptor protein]-L-lysine
• Enzyme class 4: Chain R: E.C.2.3.2.32 - cullin-RING-type E3 NEDD8 transferase.
• Reaction: S-[NEDD8-protein]-yl-[E2 NEDD8-conjugating enzyme]-L-cysteine + [cullin]- L-lysine = [E2 NEDD8-conjugating enzyme]-L-cysteine + N₆-[NEDD8- protein]-yl-[cullin]-L-lysine

Note, where more than one E.C. class is given (as above), each may correspond to a different protein domain or, in the case of polyprotein precursors, to a different mature protein.