PDBsum entry 8f3d

Ligase

PDB id: 8f3d

Contents

Protein chains

(+ 0 more) 566 a.a.

(+ 0 more) 678 a.a.

Ligands

BTI ×6

PDB id:

8f3d

Name:

Ligase

Title:

3-methylcrotonyl-coa carboxylase in filament, beta-subunit centered

Structure:

3-methylcrotonyl-coa carboxylase beta-subunit. Chain: a, b, c, d, e, f. 3-methylcrotonyl-coa carboxylase alpha-subunit. Chain: h, i, j, k, l, m

Source:

Leishmania tarentolae. Organism_taxid: 5689. Strain: tatii/uc. Strain: tatii/uc

Authors:

J.J.Hu,J.K.J.Lee,Y.T.Liu,C.Yu,L.Huang,I.Afasizheva,R.Afasizhev, Z.H.Zhou

Key ref:

J.J.Hu et al. (2023). Discovery, Structure, And function of filamentous 3-Methylcrotonyl-Coa carboxylase.. Structure, 31, 100. PubMed id: 36543169

Date:

09-Nov-22 Release date: 11-Jan-23

Protein chains

A0A640KGP9  (A0A640KGP9_LEITA) -  3-methylcrotonoyl-coA carboxylase beta subunit, putative from Leishmania tarentolae

Seq: 329 a.a.

Struc: 566 a.a.*

Protein chains

A0A640KPA4  (A0A640KPA4_LEITA) -  Methylcrotonoyl-coa carboxylase biotinylated subunitprotein-like protein from Leishmania tarentolae

Seq: 732 a.a.

Struc: 678 a.a.

* PDB and UniProt seqs differ at 30 residue positions (black crosses)

Enzyme reactions

• Enzyme class: Chains A, B, C, D, E, F: E.C.6.4.1.4 - methylcrotonoyl-CoA carboxylase.
• Reaction: 3-methylbut-2-enoyl-CoA + hydrogencarbonate + ATP = 3-methyl-(2E)- glutaconyl-CoA + ADP + phosphate + H⁺
• Cofactor: Biotin

Biotin (Bound ligand (Het Group name = BTI) matches with 93.75% similarity)

Molecule diagrams generated from .mol files obtained from the KEGG ftp site