PDBsum entry 8eic

Ligase

PDB id: 8eic

Contents

Protein chains

516 a.a.

86 a.a.

16 a.a.

Ligands

WHL

Waters ×11

PDB id:

8eic

Name:

Ligase

Title:

Crystal structure of beta-catenin and the mdm2 p53-binding domain in complex with h330, a helicon polypeptide

Structure:

Catenin beta-1. Chain: a. Synonym: beta-catenin. Engineered: yes. E3 ubiquitin-protein ligase mdm2. Chain: b. Fragment: p53 binding domain. Synonym: double minute 2 protein,hdm2,oncoprotein mdm2,ring-type e3 ubiquitin transferase mdm2,p53-binding protein mdm2.

Source:

Homo sapiens. Human. Organism_taxid: 9606. Gene: ctnnb1, ctnnb, ok/sw-cl.35, pro2286. Expressed in: escherichia coli. Expression_system_taxid: 562. Gene: mdm2. Synthetic: yes. Synthetic construct.

Resolution:

2.62Å R-factor: 0.228 R-free: 0.269

Authors:

K.Li,T.L.Travaline,J.-M.Swiecicki,O.S.Tokareva,T.M.Thomson, G.L.Verdine,J.H.Mcgee

Key ref:

K.Li et al. Recognition and reprogramming of e3 ubiquitin ligase surfaces by alpha-Helical peptides. Nat commun, . PubMed id: 37914719

Date:

14-Sep-22 Release date: 25-Oct-23

Protein chain

P35222  (CTNB1_HUMAN) -  Catenin beta-1 from Homo sapiens

Seq: 781 a.a.

Struc: 516 a.a.

Protein chain

Q00987  (MDM2_HUMAN) -  E3 ubiquitin-protein ligase Mdm2 from Homo sapiens

Seq: 491 a.a.

Struc: 86 a.a.

Protein chain

No UniProt id for this chain

Struc: 16 a.a.

Enzyme reactions

• Enzyme class: Chain B: E.C.2.3.2.27 - RING-type E3 ubiquitin transferase.
• Reaction: S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N₆- ubiquitinyl-[acceptor protein]-L-lysine