PDBsum entry 8ack

Hydrolase

PDB id: 8ack

Contents

Protein chains

468 a.a.

Ligands

GLU-ARG-TRP-GLY-HIS-ASP-PHE-ILE-LYS ×2

CAC ×2

Metals

_NA ×9

_ZN ×10

Waters ×787

PDB id:

8ack

Name:

Hydrolase

Title:

Structure of pseudomonas aeruginosa aminopeptidase, paap

Structure:

Keratinase kp1. Chain: b, a. Engineered: yes. Pcp. Chain: p, c. Engineered: yes

Source:

Pseudomonas aeruginosa. Organism_taxid: 287. Expressed in: escherichia coli. Expression_system_taxid: 562. Synthetic: yes. Organism_taxid: 287

Resolution:

1.78Å R-factor: 0.173 R-free: 0.210

Authors:

C.J.Harding,C.M.Czekster

Key ref:

C.J.Harding et al. An anti-Biofilm cyclic peptide targets a secreted aminopeptidase from p. Aeruginosa.. Nat chem biol, . PubMed id: 37386135

Date:

05-Jul-22 Release date: 12-Jul-23

Protein chains

Q9HZQ8  (LAP_PSEAE) -  Aminopeptidase from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

Seq: 536 a.a.

Struc: 468 a.a.

Enzyme reactions

• Enzyme class: E.C.3.4.11.1 - leucyl aminopeptidase.
• Reaction: Release of an N-terminal amino acid, Xaa-|-Xbb-, in which Xaa is preferably Leu, but may be other amino acids including Pro although not Arg or Lys, and Xbb may be Pro.
• Cofactor: Zn(2+)