PDBsum entry 7v8h

Ligase

PDB id: 7v8h

Contents

Protein chains

236 a.a.

Waters ×90

PDB id:

7v8h

Name:

Ligase

Title:

Crystal structure of lrr domain from shigella flexneri ipah1.4

Structure:

Ring-type e3 ubiquitin transferase. Chain: a, b. Engineered: yes

Source:

Shigella flexneri 5a str. M90t. Organism_taxid: 1086030. Strain: m90t. Gene: ipah1.4. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008.

Resolution:

2.46Å R-factor: 0.196 R-free: 0.232

Authors:

J.Liu,Y.Wang,L.Pan

Key ref:

J.Liu et al. (2022). Mechanistic insights into the subversion of the linea ubiquitin chain assembly complex by the e3 ligase ipa shigella flexneri.. Proc natl acad sci usa, 119, 76119. PubMed id: 35294289

Date:

23-Aug-21 Release date: 30-Mar-22

Protein chains

A0A0H2USG1  (A0A0H2USG1_SHIFL) -  E3 ubiquitin-protein ligase IpaH1.4 from Shigella flexneri

Seq: 575 a.a.

Struc: 236 a.a.*

* PDB and UniProt seqs differ at 1 residue position (black cross)

Enzyme reactions

• Enzyme class: E.C.2.3.2.27 - RING-type E3 ubiquitin transferase.
• Reaction: S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N₆- ubiquitinyl-[acceptor protein]-L-lysine