PDBsum entry 7txh

Hydrolase

PDB id: 7txh

Contents

Protein chains

173 a.a.

496 a.a.

292 a.a.

Ligands

GNP ×2

GOL ×16

PO4 ×4

Metals

_MG ×2

_MN ×4

Waters ×1715

PDB id:

7txh

Name:

Hydrolase

Title:

Human mras q71r in complex with human shoc2 lrr domain m173i and human pp1ca

Structure:

Ras-related protein m-ras. Chain: a, d. Synonym: ras-related protein r-ras3. Engineered: yes. Mutation: yes. Leucine-rich repeat protein shoc-2. Chain: b, e. Synonym: protein soc-2 homolog,protein sur-8 homolog. Engineered: yes.

Source:

Homo sapiens. Human. Organism_taxid: 9606. Gene: mras, rras3. Expressed in: escherichia coli. Expression_system_taxid: 562. Gene: shoc2, kiaa0862. Expressed in: spodoptera frugiperda. Expression_system_taxid: 7108.

Resolution:

1.95Å R-factor: 0.169 R-free: 0.210

Authors:

Z.J.Hauseman,J.Viscomi,A.Dhembi,K.Clark,D.A.King,M.Fodor

Key ref:

Z.J.Hauseman et al. Cooperative assembly and structure of the ras-Shoc2-P holophosphatase provides insights into the ras signal and disease-Relevant mutations. To be published, . PubMed id: 35830882

Date:

09-Feb-22 Release date: 22-Jun-22

Protein chains

O14807  (RASM_HUMAN) -  Ras-related protein M-Ras from Homo sapiens

Seq: 208 a.a.

Struc: 173 a.a.*

Protein chains

Q9UQ13  (SHOC2_HUMAN) -  Leucine-rich repeat protein SHOC-2 from Homo sapiens

Seq: 582 a.a.

Struc: 496 a.a.*

Protein chains

P62136  (PP1A_HUMAN) -  Serine/threonine-protein phosphatase PP1-alpha catalytic subunit from Homo sapiens

Seq: 330 a.a.

Struc: 292 a.a.

* PDB and UniProt seqs differ at 2 residue positions (black crosses)

Enzyme reactions

• Enzyme class 1: Chains A, D: E.C.3.6.5.2 - small monomeric GTPase.
• Reaction: GTP + H2O = GDP + phosphate + H⁺

GTP + H2O = GDP (Bound ligand (Het Group name = GNP) matches with 81.82% similarity) + phosphate + H(+) (Bound ligand (Het Group name = PO4) corresponds exactly)

• Enzyme class 2: Chains C, F: E.C.3.1.3.16 - protein-serine/threonine phosphatase.
• Reaction:
  1. O-phospho-L-seryl-[protein] + H2O = L-seryl-[protein] + phosphate
  2. O-phospho-L-threonyl-[protein] + H2O = L-threonyl-[protein] + phosphate

O-phospho-L-seryl-[protein] + H2O = L-seryl-[protein] + phosphate (Bound ligand (Het Group name = PO4) corresponds exactly)

O-phospho-L-threonyl-[protein] + H2O = L-threonyl-[protein] + phosphate (Bound ligand (Het Group name = PO4) corresponds exactly)

Note, where more than one E.C. class is given (as above), each may correspond to a different protein domain or, in the case of polyprotein precursors, to a different mature protein.

Molecule diagrams generated from .mol files obtained from the KEGG ftp site