PDBsum entry 7rof

Biosynthetic protein

PDB id: 7rof

Contents

Protein chains

385 a.a.

Ligands

TRP ×2

Metals

_NA ×4

Waters ×9

PDB id:

7rof

Name:

Biosynthetic protein

Title:

Engineered tryptophan synthase b-subunit from pyrococcus furiosus, pftrpb2b9-h275e with l-trp non-covalently bound

Structure:

Tryptophan synthase beta chain 1. Chain: a, b, c, d. Engineered: yes. Mutation: yes

Source:

Pyrococcus furiosus. Organism_taxid: 2261. Gene: trpb1, pf1706. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008

Resolution:

2.39Å R-factor: 0.227 R-free: 0.271

Authors:

P.M.Higgins,A.R.Buller

Key ref:

P.M.Higgins and a.r.buller Direct assessment of promiscuity during protein engin by substrate multiplexed screening. To be published, . PubMed id: 36068220

Date:

30-Jul-21 Release date: 03-Aug-22

Protein chains

Q8U093  (TRPB1_PYRFU) -  Tryptophan synthase beta chain 1 from Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)

Seq: 388 a.a.

Struc: 385 a.a.*

* PDB and UniProt seqs differ at 10 residue positions (black crosses)

Enzyme reactions

• Enzyme class: E.C.4.2.1.20 - tryptophan synthase.
• Pathway: Tryptophan Biosynthesis
• Reaction: (1S,2R)-1-C-(indol-3-yl)glycerol 3-phosphate + L-serine = D-glyceraldehyde 3-phosphate + L-tryptophan + H2O

(1S,2R)-1-C-(indol-3-yl)glycerol 3-phosphate + L-serine = D-glyceraldehyde 3-phosphate + L-tryptophan + H2O (Bound ligand (Het Group name = TRP) corresponds exactly)

• Cofactor: Pyridoxal 5'-phosphate

Molecule diagrams generated from .mol files obtained from the KEGG ftp site